Cytomegalo Virus Phosphoprotein 28
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 25 mM Tris-HCl pH 8.0, 1 mM EDTA and 50% glycerol)
The protein contains the CMV pp28 immunodominant regions, amino acids 130-160. The protein is purified by proprietary chromatographic technique.
Background: Human cytomegalovirus (HCMV), a member of the herpesvirus family, demonstrates cell specificity for virus assembly and release. The human cytomegalovirus pp28 is a myristylated phosphoprotein that is a constituent of the virion. The pp28 protein is positioned within the tegument of the virus particle, a protein structure that resides between the capsid and envelope. In the infected cell, pp28 is found in a cytoplasmic compartment derived from the Golgi apparatus, where the virus buds into vesicles to acquire its final membrane.
Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
Specificity: Immunoreactive with sera of CMV-infected individuals.
Jones et al. (2004) An acidic cluster of human cytomegalovirus UL99 tegument protein is required for trafficking and function. J. Virol. 78:1488.
Britt et al. (2004) Rapid genetic engineering of human cytomegalovirus by using a lambda phage linear recombination system: demonstration that pp28 (UL99) is essential for production of infectious virus. J. Virol. 78:539.
Silva et al. (2003) Human cytomegalovirus UL99-encoded pp28 is required for the cytoplasmic envelopment of tegument-associated capsids. J. Virol. 77:10594.
Wu et al. (2001) Late temporal gene expression from the human cytomegalovirus pp28US (UL99) promoter when integrated into the host cell chromosome. J. Gen. Virol. 82:1147.
Sanchez et al. (2000) Human cytomegalovirus pp28 (UL99) localizes to a cytoplasmic compartment which overlaps the endoplasmic reticulum-golgi-intermediate compartment. J. Virol. 74:3842.
Kerry et al. (1997) Translational regulation of the human cytomegalovirus pp28 (UL99) late gene. J. Virol. 71:981.