Cytomegalo Virus Phosphoprotein 28
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 25 mM Tris-HCl pH 8.0, 1 mM EDTA and 50% glycerol)
Antigen in ELISA and Western blots, excellent antigen for detection of CMV with minimal specificity problems.
The protein contains the CMV pp28 immunodominant regions, amino acids 130-160. The protein is purified by proprietary chromatographic technique.
Background: Human cytomegalovirus (HCMV), a member of the herpesvirus family, demonstrates cell specificity for virus assembly and release. The human cytomegalovirus pp28 is a myristylated phosphoprotein that is a constituent of the virion. The pp28 protein is positioned within the tegument of the virus particle, a protein structure that resides between the capsid and envelope. In the infected cell, pp28 is found in a cytoplasmic compartment derived from the Golgi apparatus, where the virus buds into vesicles to acquire its final membrane.
Specificity: Immunoreactive with sera of CMV-infected individuals.
Jones et al. (2004) An acidic cluster of human cytomegalovirus UL99 tegument protein is required for trafficking and function. J. Virol. 78:1488.
Britt et al. (2004) Rapid genetic engineering of human cytomegalovirus by using a lambda phage linear recombination system: demonstration that pp28 (UL99) is essential for production of infectious virus. J. Virol. 78:539.
Silva et al. (2003) Human cytomegalovirus UL99-encoded pp28 is required for the cytoplasmic envelopment of tegument-associated capsids. J. Virol. 77:10594.
Wu et al. (2001) Late temporal gene expression from the human cytomegalovirus pp28US (UL99) promoter when integrated into the host cell chromosome. J. Gen. Virol. 82:1147.
Sanchez et al. (2000) Human cytomegalovirus pp28 (UL99) localizes to a cytoplasmic compartment which overlaps the endoplasmic reticulum-golgi-intermediate compartment. J. Virol. 74:3842.
Kerry et al. (1997) Translational regulation of the human cytomegalovirus pp28 (UL99) late gene. J. Virol. 71:981.