recombinant, E. coli
For in vitro use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 20 mM Phosphate buffer pH 7.5)
Antigen in ELISA and Western Blots, excellent antigen for detection of West-Nile virus with minimal specificity problems.
The E. coli derived recombinant protein contains the West-Nile N-terminus Envelope Virus immunodominant regions. The protein is fused to 6xHis-tag. Mw ~42 kDa.
West Nile virus (WNV) is a virus of the family Flaviviridae part of the Japanese encephalitis (JE) antigenic complex of viruses. Image reconstructions and cryoelectron microscopyreveal a 45-50 nm virion covered with a relatively smooth proteinsurface. This structure is similar to virus, both belong to the genus flavivirus within the family Flaviviridae. WNV is a positive-sense, single strand of RNA, it is between 11,000 and 12,000 nucleotides long which encode seven non-structural proteins and three structural proteins. The RNA strand is held within a nucleocapsid formed from 12 kDa protein blocks, the capsid is contained within a host-derived membrane altered by two viral glycoproteins.
The protein is purified by proprietary chromatographic technique.
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
Saxena et al. (2013) Cloning and expression of an envelope gene of West Nile virus and evaluation of the protein for use in an IgM ELISA.Diagn Microbiol Infect Dis. 75:396.
Vogt et al. (2011) Poorly neutralizing cross-reactive antibodies against the fusion loop of West Nile virus envelope protein protect in vivo via Fcgamma receptor and complement-dependent effector mechanisms.J Virol. 85:11567.
Wang et al. (2001) West Nile virus envelope protein: role in diagnosis and immunity.Ann N Y Acad Sci. 325:325.