SARS-Associated Coronavirus Nucleocapsid
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 32 kDa
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl, 60 mM NaCl and 50% glycerol)
Recombinant SARS-ACN Antigen may be used in ELISA and Western blots, excellent for detection of SARS with minimal specificity problems.
The protein contains the Nucleocapsid protein immunodominant fragments, amino acids: 1-49. SARS-ACN is purified by proprietary chromatographic techniques.
Background: SARS (Severe Acute Respiratory Syndrome) Coronavirus is an enveloped virus containing three outer structural proteins, namely the membrane (M), envelope (E), and spike (S) proteins. The nucleocapsid (N) protein together with the viral RNA genome presumably form a helical core located within the viral envelope. The SARS-CoV nucleocapsid (N) protein is a 423 amino-acid, predicted phosphoprotein of 46 kDa that shares little homology with other members of the coronavirus family. A short serine-rich stretch, and a putative bipartite nuclear localization signal are unique to it, thus suggesting its involvement in many important functions during the viral life cycle.
Specificity: Immunoreactive with sera of SARSinfected individuals.
Liu et al. (2004) High-yield expression of recombinant SARS coronavirus nucleocapsid protein in methylotrophic yeast Pichia pastoris. World J. Gastroenterol. 10:3602.
Luo et al. (2004) Nucleocapsid protein of SARS coronavirus tightly binds to human cyclophilin A. Biochem. Biophys. Res. Commun. 321:557.
Wang et al. (2004) Low stability of nucleocapsid protein in SARS virus. Biochemistry 43:11103.
Lau et al. (2004) Detection of severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in sars patients by enzyme-linked immunosorbent assay. J. Clin. Microbiol. 42:2884.
Woo et al. (2004) Longitudinal profile of immunoglobulin G (IgG), IgM, and IgA antibodies against the severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein in patients with pneumonia due to the SARS coronavirus. Clin. Diagn. Lab. Immunol. 11:665.
Leung et al. (2004) Antibody response of patients with severe acute respiratory syndrome (SARS) targets the viral nucleocapsid. J. Infect. Dis. 190:379.