Human T cell Leukemia Virus Type-1, Envelope Protein
recombinant, E. coli
For in vitro use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 27 kDa
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 10 mM NaPO4 pH 6.0, 0.1% SDS and 1 mM DTT)
Antigen in ELISA and Western Blots. Excellent reagent for correct detection of HTLV infections with minimal specificity problems.
The protein contains the C- terminus of gp46 and most of p21E of HTLV-1. This non-fusion E. coli derived protein starts from HTLV-1 envelope amino acids 165 and ends with 440. The protein is purified by proprietary chromatographic technique.
Background: Human T-cell leukemia virus (HTLV)-1 and -2 are deltaretroviruses that infect a wide range of cells. HTLV-1 has been found primarily in CD4+ and CD8+ T-lymphocytes in vivo, whereas CD8+ T-lymphocytes are thought to be the in vivo reservoir of HTLV-2.
Specificity: Immunoreactive with all sera of HTLV-1 and HTLV-2 infected individuals with antibody response to HTLV envelope.
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
Hernandez et al. (2001) Chimeric synthetic peptides containing two immunodominant epitopes from the envelope gp46 and the transmembrane gp21 glycoproteins of HTLV-I virus. Biochem. Biophys. Res. Commun. 289:1.
Armand et al. (2000) Targeted expression of HTLV-I envelope proteins in muscle by DNA immunization of mice. Vaccine. 18:2212.
Tallet et al. (2000) Expression, purification and biological properties of the carboxyl half part of the HTLV-I surface envelope glycoprotein. J. Chromatogr. B. Biomed. Sci. Appl. 737:85.
Georges-Gobinet et al. (1998) HTLV-I associated sicca syndrome in Guadeloupe: lack of relation with a peculiar encoding sequence of surface envelope glycoprotein. Virus. Genes. 16:195.
Grange et al. (1998) Identification of exposed epitopes on the envelope glycoproteins of human T-cell lymphotropic virus type I (HTLV-I). Int. J. Cancer. 75:804.
Grange et al. (1997) Induction of neutralizing antibodies against HTLV-I envelope proteins after combined genetic and protein immunizations in mice. DNA Cell. Biol. 16:1439.
Kim et al. (2004) HTLV-1 and -2 envelope SU subdomains and critical determinants in receptor binding. Retrovirology. 1:41.