Human T cell Leukemia Virus Type-1, Envelope Protein
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 27 kDa
Purity: > 95 % (SDS-PAGE, RP-HPLC)
Form: liquid (Supplied in 10 mM NaPO4 pH 6.0, 0.1% SDS and 1 mM DTT)
Antigen in ELISA and Western Blots. Excellent reagent for correct detection of HTLV infections with minimal specificity problems.
The protein contains the C- terminus of gp46 and most of p21E of HTLV-1. This non-fusion E. coli derived protein starts from HTLV-1 envelope amino acids 165 and ends with 440. The protein is purified by proprietary chromatographic technique.
Background: Human T-cell leukemia virus (HTLV)-1 and -2 are deltaretroviruses that infect a wide range of cells. HTLV-1 has been found primarily in CD4+ and CD8+ T-lymphocytes in vivo, whereas CD8+ T-lymphocytes are thought to be the in vivo reservoir of HTLV-2.
Specificity: Immunoreactive with all sera of HTLV-1 and HTLV-2 infected individuals with antibody response to HTLV envelope.
Hernandez et al. (2001) Chimeric synthetic peptides containing two immunodominant epitopes from the envelope gp46 and the transmembrane gp21 glycoproteins of HTLV-I virus. Biochem. Biophys. Res. Commun. 289:1.
Armand et al. (2000) Targeted expression of HTLV-I envelope proteins in muscle by DNA immunization of mice. Vaccine. 18:2212.
Tallet et al. (2000) Expression, purification and biological properties of the carboxyl half part of the HTLV-I surface envelope glycoprotein. J. Chromatogr. B. Biomed. Sci. Appl. 737:85.
Georges-Gobinet et al. (1998) HTLV-I associated sicca syndrome in Guadeloupe: lack of relation with a peculiar encoding sequence of surface envelope glycoprotein. Virus. Genes. 16:195.
Grange et al. (1998) Identification of exposed epitopes on the envelope glycoproteins of human T-cell lymphotropic virus type I (HTLV-I). Int. J. Cancer. 75:804.
Grange et al. (1997) Induction of neutralizing antibodies against HTLV-I envelope proteins after combined genetic and protein immunizations in mice. DNA Cell. Biol. 16:1439.
Kim et al. (2004) HTLV-1 and -2 envelope SU subdomains and critical determinants in receptor binding. Retrovirology. 1:41.