Human Immunodeficiency Virus 1 Integrase
recombinant, E. coli
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 32 kDa
Purity: > 95 % (SDS-PAGE, HPLC)
Form: liquid (Supplied in 25 mM Tris-HCl pH 8.0, 1.5 M urea, 0.2% Triton-X and 50% glycerol)
May be used in ELISA and Western blots, excellent antigen for early detection of HIV seroconvertors with minimal specificity problems.
The E. coli derived recombinant protein is a non-glycosylated polypeptide chain, containing the HIV-1 immunodominant regions from the p31 protein (intergrase) 9-289 amino acids, fused with a GST tag at N-terminus. HIV belongs to the retrovirus family, distinguished by possession of a viral reverse transcriptase that transcribes viral RNA into DNA which is integrated into the host-cell genome. The human immunodeficiency virus (HIV) integrase (IN), one of the three virally encoded enzymes required for HIV-1 replication, must covalently join the viral cDNA into a host chromosome for productive HIV infection.
Specificity: Immunoreactive with all sera of HIV-I infected individuals.
Payan et al. (2003) [Measuring the HIV viral load with LCx (Abbott): importance for the therapeutic follow-up of 3 patients infected by type O HIV]. Transfus. Clin. Biol. 10:72.
Andreola (2004) Closely related antiretroviral agents as inhibitors of two HIV-1 enzymes, ribonuclease H and integrase: killing two birds with one stone. Curr. Pharm. Des. 10:3713.
Sechi et al. (2004) Design and synthesis of novel indole beta-diketo acid derivatives as HIV-1 integrase inhibitors. J. Med. Chem. 47:5298.
Reinke et al. (2004) L-chicoric acid inhibits human immunodeficiency virus type 1 integration in vivo and is a noncompetitive but reversible inhibitor of HIV-1 integrase in vitro. Virology 326:203.
Hazuda et al. (2004) A naphthyridine carboxamide provides evidence for discordant resistance between mechanistically identical inhibitors of HIV-1 integrase. Proc. Natl. Acad. Sci. USA 101:11233.
Aiello et al. (2004) Synthesis of novel thiazolothiazepine based HIV-1 integrase inhibitors. Bioorg. Med. Chem. 12:4459.
Mousnier et al. (2004) Nuclear import of HIV-1 integrase is inhibited in vitro by styrylquinoline derivatives. Mol. Pharmacol. 66:783.