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C-HCV

Combined Hepatitis C Virus, NS3/NS4/NS5

recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-1143 100 μg 217,30 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 70 kDa

Purity: > 95 % (SDS-PAGE)

Form: liquid (Supplied in 50 mM NaPO4 pH 8.5, 2.4 mM EDTA, 5 mM DTT and 0.1% SDS)

Applications:
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Description:
The protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions. Combined Hepatitis C Virus protein is purified by proprietary chromatographic techniques.

Background: Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then constructed into one recombinant antigen.

Specificity: Immunoreactive with sera of HCV-infected individuals.

Selected References:
Kang et al. (2005) Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein. Proteomics 5:2227.
Fukutomi et al. (2005) Hepatitis C virus core protein stimulates hepatocyte growth: Correlation with upregulation of wnt-1 expression. Hepatology 41:1096.
Gaudy et al. (2005) Usefulness of the hepatitis C virus core antigen assay for screening of a population undergoing routine medical checkup. J. Clin. Microbiol. 43:1722.
Lindh et al. (2005) Monitoring treatment response by the hepatitis C virus core antigen assay. Eur. J. Clin. Microbiol. Infect. Dis. 24:230.
Boni et al. (2005) Hepatitis C Virus Core Protein Acts as a trans-Modulating Factor on Internal Translation Initiation of the Viral RNA. J. Biol. Chem. 280:17737.