Combined Hepatitis C Virus, NS3/NS4/NS5
recombinant, E. coli
For in vitro use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 70 kDa
Purity: > 95 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM NaPO4 pH 8.5, 2.4 mM EDTA, 5 mM DTT and 0.1% SDS)
Antigen in ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.
The protein contains the HCV nucleocapsid, NS3, NS4, and NS5 immunodominant regions. Combined Hepatitis C Virus protein is purified by proprietary chromatographic techniques.
Background: Sequences from 4 gene products (proteins) of the hepatitis C virus (HCV) were scanned by using 3 different PCR-based techniques in search of the most immunoreactive regions suitable for the development of a diagnostic test for the detection of anti-HCV in human sera. All PCR fragments were cloned with pGEX4-2T expression vector and expressed in E. coli as chimeric proteins with glutathione S-transferase. The most diagnostically relevant proteins identified in this study were then constructed into one recombinant antigen.
Specificity: Immunoreactive with sera of HCV-infected individuals.
BIOZ Product Citations:
Please click the arrow on the right to expand the citation list. Click publication title for the full text.
Kang et al. (2005) Proteomic profiling of cellular proteins interacting with the hepatitis C virus core protein. Proteomics 5:2227.
Fukutomi et al. (2005) Hepatitis C virus core protein stimulates hepatocyte growth: Correlation with upregulation of wnt-1 expression. Hepatology 41:1096.
Gaudy et al. (2005) Usefulness of the hepatitis C virus core antigen assay for screening of a population undergoing routine medical checkup. J. Clin. Microbiol. 43:1722.
Lindh et al. (2005) Monitoring treatment response by the hepatitis C virus core antigen assay. Eur. J. Clin. Microbiol. Infect. Dis. 24:230.
Boni et al. (2005) Hepatitis C Virus Core Protein Acts as a trans-Modulating Factor on Internal Translation Initiation of the Viral RNA. J. Biol. Chem. 280:17737.