Recombinant Hepatitis B Surface Antigen adw subtype 2
recombinant, E. coli
For in vitro use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM arginine and 1 M urea)
Antigen in ELISA and Western blots, excellent antigen for detection of HBV with minimal specificity problems.
Recombinant HBVsAg adw antigen was mutated by replacing the Methionine residue at position 133 HBsAg is the surface antigenof the Hepatitis-B-Virus (HBV). The capsidof a virus has different surface proteins from the rest of the virus. The antigen is a protein that binds specifically on one of these surface proteins. It is commonly referred to as the Australian Antigen.
HbsAg subtype adw2 produced in E. coli, is a single, non-glycosylated, polypeptide chain containing 226 amino acids and having a molecular weight of approximately 23.0kDa. HBsAg adw2 migrates on SDS-PAGE as a 23kDa monomer band with minor amounts of dimer (46kDa) and trimer (69kDa) forms.
The HBsAg is fused to a 6 His tag on C-terminus and purified by proprietary chromatographic techniques.
Specificity: Immunoreactive with sera of HBV-infected individuals.