Cdc25A

Cell division cycle phosphatase

human, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-348 20 μg 218,50 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 68 kDa

Accession number: M81933

Accession number: M81933

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl and 2 mM DTT)

pH: 7.2

Activity: 1 nmol of Cdc25A will hydrolyze 10 nmol of pNPP per minute at 37°C at pH 8.0.

Description:
The dual specific Cdc25A (Cell division cycle) phosphatase regulates cell cycle progression, has oncogenic and anti-apoptotic activity, and is overexpressed in many human tumors. The protein does not have tags.

Assay buffer: 50 mM Tris-HCl, pH 8.0, 50 mM NaCl, 0.1 mM EDTA.

Selected References:
Fauman et al. (1998) Crystal structure of the catalytic domain of the human cell cycle control phosphatase, Cdc25A. Cell 93:617.
Gottlin et al. (1996) Kinetic analysis of the catalytic domain of human cdc25B. J. Biol. Chem. 271:27445.