Ras-proximate 1B, deletion of 17 C-terminal residues
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 19.0 kDa (167 amino acids)
Accession number: NM_015646
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 64 mM Tris-HCl pH 7.0, 5 mM DTE, 400 mM NaCl, 10 mM MgCl2 and 0.1 mM GDP)
Rap (Ras-proximate) proteins are GTPases that belong to the Ras superfamily of small GTP binding proteins. Rap1B is known to antagonize the mitotic and transforming activity of Ras. Rap1B can be activated by cAMP known to either stimulate or inhibit cell proliferation. The deletion of 17 C-terminal amino acids in Rap1B ΔC leads to the loss of the phosphorylation site Ser179 and the geranyl-geranylation site. Protein preparation is 53% GDP- and 47% GTP-loaded, measured by HPLC.
Ribeiro-Neto et al. (2002) On the mitogenic properties of Rap1b:cAMP-induced G (1)/S entry requires activated and phosphorylated Rap1b. Proc. Natl. Acad. Sci. USA 99:5418.
Altschuler et al. (1998) Mitogenic and oncogenic properties of the small G protein Rap1b. Proc. Natl. Acad. Sci. USA 95:7475.
Miura et al. (1992) Phosphorylation of smg p21B/rap1B p21 by cyclic GMP-dependent protein kinase. FEBS Lett. 297:171.