Ran - E70A

For general laboratory use.

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 24.0 kDa (216 amino acids)

Accession number: NM_006325

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 20 mM KH₂PO₄/K₂HPO₄ pH 7.4, 1 mM DTE and 5 mM MgCl₂)

Description:
Ran (Ras-related nuclear protein, TC4) belongs to the Ras superfamily of GTP binding proteins. It acts in cellular processes such as nuclear transport, mitotic spindle assembly, and post-mitotic nuclear assembly. The mutation E70A results in inhibition of the guanine exchange reaction mediated by RCC1, the guanine nucleotide exchange factor (GEF) for Ran. Protein preparation is 85% GDP-loaded, measured by HPLC.

BIOZ Product Citations:

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Selected References:
Dasso (2002) The Ran GTPase: Theme and Variations. Current Biology 12:502.
Renault et al. (2001) Structural Basis for Guanine Nucleotide Exchange on Ran by the regulator of Chromosome Condensation (RCC1). Cell 105:245.