» Sign in / Register

Rab24GST-His

Ras-associated, small GTP-binding protein

mouse, recombinant, E. coli

Cat. No. Amount Price (EUR) Buy / Note
PR-120 50 μg 212,18 Add to Basket/Quote Add to Notepad

For in vitro use only!

Shipping: shipped on dry ice

Storage Conditions: store at -80 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Molecular Weight: 52 kDa

Accession number: NM_009000

Accession number: NM_009000

Purity: > 90 % (SDS-PAGE)

Form: liquid (Supplied in 25 mM HEPES pH 7.2, 40 mM NaCl, 2 mM MgCl2, 10 μM GDP and 3 mM DTE)

Activity: 100 pmol of protein can bind > 80 pmol of GDP.

Description:
N-terminal tagged Rab24 is a small GTPase that belongs to the Ras superfamily. Rab proteins play an important role in various aspects of membrane traffic, including cargo selection, vesicle budding, vesicle motility, tethering, docking, and fusion. Rab24 possesses several unusual characteristics that distinguish it from other Rab proteins. Rab24 exists predominantly in the GTP state when expressed in cultured cells. The low GTPase activity is related to the presence of serine instead of glutamine at the position cognate to Ras Gln-61. Rab24 was found in the endoplasmic reticulum/cis-Golgi region and on late endosomal structures. The localization of Rab24 may indicate its involvement in autophagyrelated processes. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.

Selected References:
Stenmark et al. (2001) The Rab GTPase family. Genome Biol. 2:30071.
Somsel et al. (2000) Rab GTPases coordinate endocytosis. J. Cell Sci. 113:183.
Erdman et al. (2000) Rab24 Is an Atypical Member of the Rab GTPase Family. Deficient GTPase activity, GDP dissociation inhibitor interaction, and prenylation of Rab24 expressed in cultured cells. J. Biol. Chem. 275:3848.
Olkkonen et al. (1993) Molecular cloning and subcellular localization of three GTP-binding proteins of the rab subfamily. J. Cell Sci. 106:1249.