Ras-associated, small GTP-binding protein
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 53 kDa
Accession number: NM_004578
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 50 mM Tris-HCl pH 8.0, 200 mM NaCl, 10 mM MgCl2 and 2 mM beta-mercaptoethanol)
Activity: 100 pmol of protein can bind > 80 pmol of GDP.
Rab4 is a small GTPase that belongs to the Ras superfamily. Rab proteins play an important role in various aspects of membrane traffic, including cargo selection, vesicle budding, vesicle motility, tethering, docking, and fusion. The monomeric GTPase Rab4 is associated with early endosomes and regulates recycling vesicle formation. Together with Rab5 it act to control influx and efflux out of early endosomes. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.
Chamberlain et al. (2008) Disrupted RabGAP Function of the p85 Subunit of Phosphatidylinositol 3-Kinase Results in Cell Transformation. J. Biol. Chem. 283:15861.
Stenmark et al. (2001) The Rab GTPase family. Genome Biol. 2:30071.
Mohrmann et al. (2002) Rab4 function in membrane recycling from early endosomes depends on a membrane to cytoplasm cycle. J. Biol. Chem. 277:32029.
Somsel et al. (2000) Rab GTPases coordinate endocytosis. J. Cell Sci. 113:183.
Chamberlain et al. (2004) The p85alpha Subunit of Phosphatidylinositol 3-Kinase Binds to and Stimulates the GTPase Activity of Rab Proteins. J. Biol. Chem. 279 (47):48607.