Ras Binding Domain of Raf, c-Raf 1 Kinase
human, recombinant, E. coli
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
thaw quickly/avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 42 kDa
Accession number: X03484 / P04049
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in PBS, pH 7.4 )
Raf is a member of the serine/threonine proteine kinase family involved in regulation of cell growth and differentiation and is the most important effector of Ras. Full-length Raf is composed of three conserved regions responsible for interaction with Ras, for phosphorylation and for catalytic activity. RafRBD (Ras binding domain, amino acids 50-132) mediates interaction with membrane-anchored Ras necessary for activation of the kinase activity of Raf. The GST-Tag facilitates the protein`s application in typical GST pull-down assays.
The protein is GST-tagged on its N-terminus. There is a 7 AA linker between GST and RBD.
Reommended thawing and freezing :
Thaw the protein quickly at approx. 30 °C in a water bath and refreeze aliquots fast on dry ice.
Eing et al. (2002) Quantification of the Raf-C1 interaction with solidsupported bilayers. ChemBioChem 3:190.
Emerson et al. (1996) Structure of Ras-binding domain of c-Raf-1 as determined by NMR spectroscopy and identification of the region that interacts with Ras. Drug Des. Discov. 13:83.
Nasser et al. (1995) The 2.2 crystal structure of the Ras-binding domain of the serine/threonine kinase c-Raf1 in complex with Rap1A and a GTP analogue. Nature 375:554.