Ras Binding Domain of the Ral Guanine Nucleotide Dissociation Stimulator
human, recombinant, E. coli,
Recognized GTPases: Rap-1A, Rap-1B, Rap-2A, Rap-2B, Rit, Rin
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Molecular Weight: 40 kDa
Accession number: NM_006266
Purity: > 90 % (SDS-PAGE)
Form: liquid (Supplied in 30 mM Tris-HCl pH 7.5, 100 mM NaCl, 5 mM MgCl2 and 5 mM DTE)
Ras-binding domain (RBD) of the human RalGDS, comprises amino acids 788-885 (97aa) of wild type. RalGDS (Ral-guanine nucleotide dissociation stimulator) is the guanine nucleotide exchange factor (GEF) for the Ras-related protein Ral. The GEF-activity of RalGDS is stimulated by binding of Ras. The GST-tag facilitates the protein`s application in typical GST pull down assays.
Vetter et al. (1999) Structural and biochemical analysis of Raseffector signaling via RalGDS. FEBS Letters 451:175.
Murphy et al. (2002) Involvement of phosphatidylinositol 3-kinase, but not RalGDS, in TC21/R-Ras2-mediated transformation. J. Biol. Chem. 277:9966.