native, HEL-299 cell culture
For in vitro use only!
Shipping: shipped on dry ice
Storage Conditions: store at -80 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied in 0.1 M glycine buffer pH 9.5)
Culture system: HEL-299 cell culture
Protein concentration: Lot specific. Determined by Bradford assay (Biorad) using BSA as standard.
Preparation: Infected cells are harvested, washed and virus extracted by alkaline treatment and sonication. The resulting antigen preparation consists of a high concentration of virus and viral components as wellas some cellular material suspended in 0.1 M glycine buffer, pH9.5.
Inactivation: This product has been treated with Beta-propiolactone. Confirmed inactive by attempted growth under original culture conditions. Since no procedure can guarantee absolute sterility, the reagent should be handled with appropriate safety precautions.