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anti-Head-Peptide

rabbit, polyclonal

Cat. No. Amount Price (EUR) Buy / Note
ABD-058 20 μl 153,75 Add to Basket/Quote Add to Notepad
Fig. 1: Antenna heart: The immunoreactivity (ir) of head peptide (green) is different to allatostatin-ir (red, MAB)
Fig. 1: Antenna heart: The immunoreactivity (ir) of head peptide (green) is different to allatostatin-ir (red, MAB)
Fig 2 Antenna heart The immunoreactivity (ir) of head peptide (green) appear in the same nerve fiber
Fig. 2: Antenna heart: The immunoreactivity (ir) of head peptide (green) appear in the same nerve fiber as the octopamine-ir (red, MAB)

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid (Supplied as serum, preserved in glycerol (50 % saturation))

Applications:
Anti-Head-Peptide can be used for ELISA and Immunocytochemistry.

Description:
The anti-head peptide serum was raised against the Periplaneta americana midgut neuropeptide, ANRSPSLRLRLRFamide, coupled to bovine thyroglobulin using glutaraldehyde.

Specificity:
Antisera to FMRFamide often only need the last two amino acids (RFamide) for recognition. In insects the FMRFamide-related peptides (FaRPs) are grouped into different families, which are expressed from different precursor genes: the FMRFamides), the myosuppressins, the sulfakinins and the head peptides. A crossreactivity of the anti-head peptide serum to the other FaRPs is visible, but the pattern of the head peptide immunoreactivity from brain, ventral nerve cord, midgut and antenna-heart (Fig 1, 2) is different to tested RF-amide sera (FMRFamide, anti-Perisulfakinin).

Selected References:
Predel et al. (2004) Unique accumulation of neuropeptides in an insect: FMRFamide-related peptides in the cockroach, Periplaneta americana. Eur J Neurosci. 20:1499.
Veenstra et al. (1995) Isolation of a novel RFamide peptide from the midgut of the American cockroach, Periplaneta americana Biochem Biophys Res Commun. 15,213:519.
Veenstra et al. (1995) Immunohistological localization of regulatory peptides in the midgut of the female mosquito Aedes aegypti Histochem Cell Biol. 104:337.