For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied as serum, preserved in glycerol (50 % saturation))
Anti-Head-Peptide can be used for ELISA and Immunocytochemistry.
The anti-head peptide serum was raised against the Periplaneta americana midgut neuropeptide, ANRSPSLRLRLRFamide, coupled to bovine thyroglobulin using glutaraldehyde.
Antisera to FMRFamide often only need the last two amino acids (RFamide) for recognition. In insects the FMRFamide-related peptides (FaRPs) are grouped into different families, which are expressed from different precursor genes: the FMRFamides), the myosuppressins, the sulfakinins and the head peptides. A crossreactivity of the anti-head peptide serum to the other FaRPs is visible, but the pattern of the head peptide immunoreactivity from brain, ventral nerve cord, midgut and antenna-heart (Fig 1, 2) is different to tested RF-amide sera (FMRFamide, anti-Perisulfakinin).
Predel et al. (2004) Unique accumulation of neuropeptides in an insect: FMRFamide-related peptides in the cockroach, Periplaneta americana. Eur J Neurosci. 20:1499.
Veenstra et al. (1995) Isolation of a novel RFamide peptide from the midgut of the American cockroach, Periplaneta americana Biochem Biophys Res Commun. 15,213:519.
Veenstra et al. (1995) Immunohistological localization of regulatory peptides in the midgut of the female mosquito Aedes aegypti Histochem Cell Biol. 104:337.