For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Form: liquid (Supplied as serum and glycerol) These antisera are obtained from rabbits after immunization with AIKETERHKCR peptide motif (Biotrend, Colgne, Germany).
Immunocytochemistry, ELISA and Western Blots.
Polyclonal antibody to insect Perisulfakinin Receptor.
RT-PCR and Western blot analysis of PSKR expression. (A) Nested RT-PCR analysis of the PSKR expression in brain and ganglia. A 362 bp PCR product was amplified in all neuronal samples tested. (B) Single-cell RT-PCR analysis of isolated DUM neurons. In 7 out of 10 DUM neurons a PSKR-specific band was found (upper panel). As an endogenous expression control an actin-specific RT-PCR was used (lower panel). M, marker pBR322 DNA BsuRI digested (two marker bands are indicated). (C) Western blot analysis of PSKR expression in nervous system (NS) and gut (G). A 68 kDa band was found in gut only.
Synthetic peptide motif AIKETERHKCR (Biotrend, Colgne, Germany)located in the loop between TMS4 and TMS5.
Wicher et al. (2007) The satiety signaling neuropeptide perisulfakinin inhibits the activity of central neurons promoting general activity. Front Cell Neurosci. 1:3 Epub.