EDA-GppNHp (EDA-GMPPNP)-ATTO-488

excitation and emission spectrum of ATTO 488

For general laboratory use.

Shipping: shipped on dry ice

Storage Conditions: store at -20 °C

Shelf Life: 6 months after date of delivery

Molecular Formula: C₃₈H₄₄N₁₁O₂₃P₃S₂ (free acid)

Molecular Weight: 1179.87 g/mol (free acid)

Exact Mass: 1179.13 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: yellow

Concentration: 1.0 mM - 1.1 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ_exc 500 nm, λ_em 520 nm, ε 90.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Please note: For reasons of stability, please make sure that the pH value of a solution of this product never drops below 7.0. This can be achieved by dissolving the nucleotide in a buffer of your choice (50 - 100 mM, pH 7 - 10). Dissolve and adjust concentration photometrically.

BIOZ Product Citations:

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Selected References:
Lee et al. (2017) Mechanism of SOS PR-domain autoinhibition revealed by single-molecule assays on native protein from lysate. Nat. Commun. 8:15061.
Christensen et al. (2016) One-way membrane trafficking of SOS in receptor-triggered Ras activation. Nat. Struct. Mol. Biol. 23 (9):838.
Lin et al. (2014) H-Ras forms dimers on membrane surfaces via a protein-protein interface. Proc. Natl. Acad. Sci. USA 111 (8):2996.