XTP - Triethylammonium salt
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C10H15N4O15P3 (free acid)
Molecular Weight: 524.17 g/mol (free acid)
Exact Mass: 523.97 g/mol (free acid)
CAS#: 6253-56-1
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 276 nm, ε 9.6 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Applications:
Inhibitor of adenylyl and guanylyl cyclase[1]
Specific Ligands:
Soluble guanylyl cyclase[2]
Related products: XTP, Sodium salt #NU-935
Selected References:
[1] Spangler et al. (2011) Interaction of the diguanylate cyclase YdeH of Escherichia coli with 2', (3')-substituted purine and pyrimidine nucleotides. J. Pharmacol. Exp. Ther. 336 (1):234.
[2] Chang et al. (2005) Nitric Oxide-dependent Allosteric Inhibitory Role of a Second Nucleotide Binding Site in Soluble Guanylyl Cyclase. J. Biol. Chem. 280 (12):11513.
Beste et al. (2012) Nucleotidyl cyclase activity of soluble guanylyl cyclase α1β1. Biochemistry 51 (1):194.
Spangler et al. (2011) Interaction of the diguanylate cyclase YdeH of Escherichia coli with 2', (3')-substituted purine and pyrimidine nucleotides. J Pharmacol Exp. Ther. 336 (1):234.
Fulga et al. (2001) SR beta coordinates signal sequence release from SRP with ribosome binding to the translocon. EMBO J. 20 (9):2338.
Legate et al. (2000) Nucleotide-dependent binding of the GTPase domain of the signal recognition particle receptor betasubunit to the alpha-subunit. J. Biol. Chem. 275 (35):27439.
Sakash et al. (2000) The use of nucleotide analogs to evaluate the mechanism of the heterotropic response of Escherichia coli aspartate transcarbamoylase. Protein Sci. 9 (1):53.
Hwang et al. (1999) Structure-based identification of a novel NTPase from Methanococcus jannaschii. Nat. Struct. Biol. 6 (7):691.
Muraoka et al. (1999) Effects of purinenucleotide analogues on microtubule assembly. Cell Struct. Funct. 24 (5):305.
Seifert et al. (1999) Effects of guanine, inosine, and xanthine nucleotides on beta (2)-adrenergic receptor/G (s) interactions: Evidence for multiple receptor conformations. Mol. Pharmacol. 56 (2):348.
Yu et al. (1997) Characterization of a Go alpha mutant that binds xanthine nucleotides. J. Biol. Chem. 272 (29):18015.
Rybin et al. (1996) GTPase activity of Rab5 acts as a timer for endocytic membrane fusion. Nature 383 (6597):266.
Powers et al. (1995) Reciprocal stimulation of GTP hydrolysis by 2 directly interacting GTPases. Science 269 (5229):1422.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-602S | 15 μl (100 mM) | 165,40 € |
| NU-602L | 5 x 15 μl (100 mM) | 484,60 € |
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Scientific citations for Jena Bioscience nu-602
3 scientific citations and 0 citation images found by Bioz.
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Ancestral [Fe-S] biogenesis system SMS has a unique mechanism of cluster assembly and sulfur utilization.
PLoS Biology, 2025-06-25 · PMID:40560930
For Kd measurement, a concentration range of SmsC proteins from 0 to 40 μM and SmsC K45R proteins from 0 to 25 μM were incubated with a fixed 400 nM concentration of man- tATPyS (2′/3′-O-(N-Methyl-anthraniloyl)-adenosine-5′-(γ-thio)-triphosphate,
Triethylammonium salt fromJena Bioscience λ max 255/355 nm, ɛ 23.3/5.8 L mmol−1 cm−1 (Tris-HCl pH 7.5), λ exc 355 nm, λ em 448 nm) [46] in HEPES buffer 25 mM, KCl 100 mM, MgSO 4 5 mM, pH 7.6. -
Deubiquitinase OTUD6B Isoforms are Important Regulators of Growth and Proliferation
Molecular cancer research : MCR, 2018-02-01 · PMID:27864334
Cap binding assay was performed after transfection with control siRNA or siRNA against OTUD6B as previously described ( 20 ) with one modification: initiation complexes were formed using 2’/3’-O-(2-Aminoethyl-carbamoyl)-N7-methyl-guanosine-5’-triphosphate-Biotin,
Triethylammonium salt (Jena Bioscience ) and captured on streptavidin magnetic beads (Pierce). -
Substrate binding disrupts dimerization and induces nucleotide exchange of the chloroplast GTPase Toc33
Biochemical Journal, 2011-05-13 · DOI:10.1042/bj20110246
.. performed as described previously [19].Protein expression and purification was performed as described previously [19].. EDA-GTP-ATTO-550 [2′/3′-O-(2-aminoethylcarbamoyl)-GTP, triethylammonium salt] and mantGDP [2′/3′- O-(N-methylanthraniloyl)-GDP,
triethylammonium salt ] were obtained fromJena Bioscience .. All calculations were preformed with SigmaPlot (SPSS).All calculations were preformed with SigmaPlot (SPSS).
