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Thermolabile UNG (Uracil N-Glycosylase) - 10 u/μl

UNG (UDG) for preventing carry-over contaminations in PCR assays

Cat. No. Amount Price (EUR) Buy / Note
PCR-427-1KU 1 kilo unit 542,00 Add to Basket/Quote Add to Notepad
PCR-427-10KU 10 kilo units 2.710,00 Add to Basket/Quote Add to Notepad

For general laboratory use.

Unit Definition: One unit of enzyme catalyzes the degradation of 1 μg single-stranded uracil-containing DNA at 37 °C in 60 min.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Form: liquid

Concentration: 10 unit/μl

Thermolabile UNG (UDG) is used in real-time PCR to prevent carry-over contamination of dU-containing DNA from previous reactions. The enzyme is fully active at temperatures up to 55°C and inactivated above 65°C.
Thermolabile Uracyl N-Glycosylase (UNG, UDG) catalyzes the release of uracil from single and double stranded uracyl-containing DNA. The resulting abasic sites are susceptible to hydrolytic cleavage at elevated temperatures.
An amount of 1 unit UNG completely digests 104 to 106 copies of U-containing DNA fragments in 2 min at 50°C.

Recommended assay:
Use 0.2 units UNG per PCR assay (50 μl volume).
An UNG treatment of 2 min at 50°C at the onset of thermal cycling removes uracil residues from dU-containing DNA and prevents it from serving as template. UNG is completely heat-inactivated at temperatures above 65°C in the following initial denaturation step of the PCR.

10 units/μl UNG in 20 mM Tris-HCl pH 8.0, 50 mM NaCl, 1 mM EDTA, 1 mM DTT, 100 μg/ml BSA, 50 % [v/v] Glycerol

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