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dNTP Mix - 10 mM Solution

Equimolar Mix of 10 mM dATP, dCTP, dGTP and dTTP

2'-Deoxyadenosine-5'-triphosphate, Sodium salt; 2'-Deoxycytidine-5'-triphosphate, Sodium salt; 2'-Deoxyguanosine-5'-triphosphate, Sodium salt; 2'-Deoxythymidine-5'-triphosphate, Sodium salt

Cat. No. Amount Price (EUR) Buy / Note
NU-1006S 1 ml 60,40 Add to Basket/Quote Add to Notepad
NU-1006L 5x 1 ml 242,00 Add to Basket/Quote Add to Notepad
NU-1006-10ML 10 ml 299,90 Add to Basket/Quote Add to Notepad
For 100 ml bulk amounts, customized fillings or individual formulations please request your customized quotation at info@jenabioscience.com.

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible. If stored as recommended, Jena Bioscience guarantees optimal performance of this product for 12 months after date of delivery.

Shelf Life: 12 months

Molecular Formula:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dTTP: C10H17N2O14P3 (free acid)

Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)

Purity: ≥ 99 % (HPLC)

Form: clear aqueous solution

pH: 8.5 ±0.2 (22 °C)

For standard PCR applications a final concentration of 200 μM each dNTP is recommended.

dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).

Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable

Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.