dNTP Mix - 10 mM Solution
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
dNTP Mix is an equimolar mixture of ultrapure dATP, dCTP, dGTP, and dTTP supplied as clear aqueous solution (pH 8.5).
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Molecular Formula:
dATP: C10H16N5O12P3 (free acid)
dCTP: C9H16N3O13P3 (free acid)
dGTP: C10H16N5O13P3 (free acid)
dTTP: C10H17N2O14P3 (free acid)
Molecular Weight:
dATP: 491.18 g/mol (free acid)
dCTP: 467.15 g/mol (free acid)
dGTP: 507.18 g/mol (free acid)
dTTP: 482.17 g/mol (free acid)
Purity: ≥ 99 % (HPLC)
Form: clear aqueous solution
pH: 8.5 ±0.2 (22 °C)
Applications:
For standard PCR applications a final concentration of 200 μM each dNTP is recommended.
Quality Control Specifications:
Low Copy Long Range PCR (18 kb, lambda DNA, template dilution series): PCR fragment with 50 pg of template or less
RT-PCR (749 bp fragment, human GAPDH gene, template dilution series): PCR fragment with 10 pg of template or less
Contamination with bacterial or human DNA: not detectable
DNases, RNases, Nicking Activity: not detectable
Proteases: not detectable
Selected References:
Erlich et al. (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 29 (239):487.
Holland et al. (1991) Detection of specific polymerase chain reaction product by utilizing the 5'----3' exonuclease activity of Thermus aquaticus DNA polymerase. Proc. Natl. Acad. Sci. USA 88 (16):7276.
Sanger et al. (1977) DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA 74:5463.
| Cat. No. | Amount | Price |
|---|---|---|
| NU-1006S | 1 ml | 67,50 € |
| NU-1006L | 5x 1 ml | 270,40 € |
| NU-1006-10ML | 10 ml | 314,70 € |
| NU-1006-100ML | 100 ml | Ask for Quotation |
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Scientific citations for Jena Bioscience nu-1006
27 scientific citations and 1 citation images found by Bioz.
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Sex-specific molecular and cellular phenotypes during resolution of neuropathic pain in dorsal root ganglia.
Cell Reports, 2026-05-28 · PMID:42207640
.. ..Aquapolymount Polyscienes Inc. #18606 DAPI Sigma-Aldrich #D9542
dNTP Mix 10mMJena Bioscience #NU-1006 S Donkey Serum Sigma-Aldrich #D9663 Heparin-Natrium Ratiopharm.. .. -
Unveiling Metabolic Subtypes in Endometrial Cancer Cell Lines: Insights from Metabolomic Analysis Under Standard and Stress Conditions.
International Journal of Molecular Sciences, 2025-10-16 · PMID:41096838
The reaction mixture consisted of 5 μL of 5× RT buffer (in house), 1 μL of reverse transcriptase primer (in house),
1 μL ofdNTPs (Jena Bioscience , Jena, Germany), 0.25 μL of Maxima Reverse Transcriptase (Thermo Fisher Scientific, Waltham, MA, USA), 0.25 μL of SUPERase·In RNase inhibitor (Invitrogen, CA, USA) and nuclease-free water to a final volume of 25 μL. -
Chemotranscriptomic profiling with a thiamine monophosphate photoaffinity probe
Chemical Science, 2025-02-11 · DOI:10.1039/d4sc06189f
.. mM), five-fold first-strand buffer (4 μL), and DTT (0.1 M, 1 μL) were added.. To create a sequencing lane, RT was performed using a
dNTP mix containing a10:1 molar ratio for one selected ddNTP:dNTP (Jena Biosciences ), whereas the other three dNTPs are in 1:1 molar ratio to the selected ddNTP.. Superscript III was added to initiate all RT reactions.Superscript III was added to initiate ..
