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ROX Reference Dye - 100 μM

Reference dye for fluorescence signal normalization

Cat. No. Amount Price (EUR) Buy / Note
PCR-356-1ML 1 ml (100 μM)112,00 Add to Basket/Quote Add to Notepad
PCR-356-10ML 10 ml (100 μM)526,00 Add to Basket/Quote Add to Notepad
PCR-356-100ML 100 ml (100 μM)2.630,00 Add to Basket/Quote Add to Notepad

For general laboratory use.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months

Form: liquid

Color: pink

Concentration: 100 μM

Spectroscopic Properties: λexc 576 nm, λem 601 nm

ROX reference dye is recommended for block-based real-time PCR instruments that are compatible with the evaluation of a ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 100 μM concentration.

100 μM ROX dye in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20

Preparation of the qPCR master mix with ROX
Preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly before use.

assay set-upfinal ROX concentration
low ROX assay50 nM
high ROX assay500 nM

Related products:

  • qPCR Core Kits
  • Dual labeled fluorescent probes
  • Custom primers