Reference dye for fluorescence signal normalization
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Shelf Life: 12 months
Concentration: 100 μM
Spectroscopic Properties: λexc 576 nm, λem 601 nm
ROX reference dye is recommended for block-based real-time PCR instruments that are compatible with the evaluation of a ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 100 μM concentration.
100 μM ROX dye in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
Preparation of the qPCR master mix with ROX
Preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly before use.
|assay set-up||final ROX concentration|
|low ROX assay||50 nM|
|high ROX assay||500 nM|