Optimized buffer system for real-time PCR
For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C or -20 °C
Shelf Life: 12 months
Concentration: 10x conc.
qPCR Buffer is optimized for use in real-time PCR applications. The buffer contains a well-balanced ratio of potassium-, ammonium- and magnesium-ions to ensure high specificity and minimal by-product formation in probe-based assays as well as in SybrGreen/EvaGreen-based assays. There is no additional optimization required for most assay set-ups.
qPCR Buffer (green cap)
200 mM Tris-HCl, KCl, (NH4)2SO4 and 30 mM MgCl2.
Optimization of MgCl2 concentration:
MgCl2 Solution - 25 mM (#PCR-266) is recommended for optimization of the final Mg2+ concentration. A concentration of 2 - 5 mM is required for optimal functionality. Lower concentrations give higher specificity, whereas higher concentrations generate a higher yield.