» Sign in / Register

qPCR Buffer

Optimized buffer system for real-time PCR

Cat. No. Amount Price (EUR) Buy / Note
PCR-279S 5 x 1,2 ml17,10 Add to Basket/Quote Add to Notepad
PCR-279-10ML 10 ml24,20 Add to Basket/Quote Add to Notepad
PCR-279-100ML 100 ml120,80 Add to Basket/Quote Add to Notepad

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at 4 °C or -20 °C

Shelf Life: 12 months

Form: Liquid

Concentration: 10x conc.

qPCR Buffer is optimized for use in real-time PCR applications. The buffer contains a well-balanced ratio of potassium-, ammonium- and magnesium-ions to ensure high specificity and minimal by-product formation in probe-based assays as well as in SybrGreen/EvaGreen-based assays. There is no additional optimization required for most assay set-ups.

qPCR Buffer (green cap)
200 mM Tris-HCl, KCl, (NH4)2SO4 and 30 mM MgCl2.

Optimization of MgCl2 concentration:
MgCl2 Solution - 25 mM (#PCR-266) is recommended for optimization of the final Mg2+ concentration. A concentration of 2 - 5 mM is required for optimal functionality. Lower concentrations give higher specificity, whereas higher concentrations generate a higher yield.