For general laboratory use.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C or -20 °C
Shelf Life: 12 months
Concentration: 10x conc.
Ruby PCR Buffer contains gel loading buffer and an inherent red dye allowing the direct loading of the PCR product into the gel. The red dye allows an easy visual control during PCR set-up and in combination with the density reagent the direct loading of the reaction product into the gel.
The buffer guarantees robust and reliable amplification results in almost all PCR applications. It contains a well-balanced ratio of potassium-, ammonium- and magnesium-ions to ensure high specificity and minimal by-product formation without the need of additional optimization steps.
Ruby PCR Buffer (green cap)
200 mM Tris-HCl, KCl, (NH4)2SO4 and 20 mM MgCl2 red dye and density reagent
Optimization of MgCl2 concentration:
A final Mg2+ concentration of 2.0 mM is recommended for most PCR applications. For an individual optimization of the reaction buffer add 25 mM MgCl2 stock solution (PCR-266) as shown in the table below.
|final MgCl2 conc.||20 μl final assay volume||50 μl final assay volume|
|3 mM||0.8 μl||2.0 μl|
|4 mM||1.6 μl||4.0 μl|
|5 mM||2.4 μl||6.0 μl|
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