KCl Buffer
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
PCR Buffer KCl is recommended for use in routine PCR reactions. It is optimized for high specificity and guarantees minimal by-product formation.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C or -20 °C
Shelf Life: 12 months
Form: Liquid
Concentration: 10x conc.
Content:
PCR Buffer KCl (green cap)
200 mM Tris-HCl (pH 8.5, 25 °C), 500 mM KCl and 15 mM MgCl2.
Optimization of MgCl2 concentration:
A final Mg2+ concentration of 1.5 mM is recommended for most PCR applications if using PCR Buffer KCl. For an individual optimization of the reaction buffer add 25 mM MgCl2 stock solution (PCR-266) as shown in the table below.
| final MgCl2 conc. | 20 μl final assay volume | 50 μl final assay volume |
|---|---|---|
| 1.5 mM | - | - |
| 2 mM | 0.4 μl | 1.0 μl |
| 3 mM | 1.2 μl | 3.0 μl |
| 4 mM | 2.0 μl | 5.0 μl |
| 5 mM | 2.8 μl | 7.0 μl |
| Cat. No. | Amount | Price |
|---|---|---|
| PCR-262S | 5 x 1,2 ml | 16,70 € |
| PCR-262-10ML | 10 ml | 25,00 € |
| PCR-262-100ML | 100 ml | 125,00 € |
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Scientific citations for Jena Bioscience pcr-262
24 scientific citations and 11 citation images found by Bioz.
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NuMA is a mitotic adaptor protein that activates dynein and connects it to microtubule minus ends
The Journal of Cell Biology, 2025-04-07 · PMID:39932518
1 μM of test protein was mixed with stabilized microtubules for microtubule sedimentation assays (0.5 μM polymerized tubulin) in
NuMA microscopy buffer (BRB80,50 mM KCl,1 mM MgCl 2 , 1 mM EGTA, 2 mM guanosine-5′-triphosphate [GTP] [Jena Bioscience ], 0.15% [wt/vol] methylcellulose, 1% [wt/vol] glucose, 1 mM TCEP).. The appropriate amount of KCl was supplemented to achieve a .. -
Cysteine polysulfidation and mitochondrial bioenergetics regulated by cysteinyl-tRNA synthetase
, 2024-01-09 · DOI:
The ribosomal fraction was suspended in
polysome buffer (50 mM Tris-HCl, pH 7.5, 10 mM MgCl2, and 25 mM KCl), containing an EDTA-free protease inhibitor cocktail (as indicated by the manufacturer), and was then reacted with 2 mM IAM at room temperature for 30 min. After the ribosomal fraction was washed with the poly-some buffer, the ribosomes were treated with 5′-biotin-dC-puromycin (Jena Bioscience , Jena, Germany) in TTBS (20 mM Tris-HCl, 150 mM NaCl, .. -
OPA1 helical structures give perspective to mitochondrial dysfunction.
Nature, 2023-09-14 · PMID:37612506
Basal GTP hydrolysis of s-OPA1 was measured using the colorimetric malachite-green assay described previously58 Reactions were performed at 37 °C using s-OPA1 at
2 μM inHCB150 buffer (20 mM HEPES,pH 7.2 , 150 mM KCl,1 mM MgCl2,2 mM EGTA and 1 mM DTT) in the presence of GTP (Jena Biosciences ).
