Reference dye for fluorescence signal normalization
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| PCR-356-1ML | 1 ml (100 μM) | 112,00 | Add to Basket/Quote Add to Notepad |
| PCR-356-10ML | 10 ml (100 μM) | 526,00 | Add to Basket/Quote Add to Notepad |
| PCR-356-100ML | 100 ml (100 μM) | 2.630,00 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
store dark
Shelf Life: 12 months
Form: liquid
Color: pink
Concentration: 100 μM
Spectroscopic Properties: λexc 576 nm, λem 601 nm
Description:
ROX reference dye is recommended for block-based real-time PCR instruments that are compatible with the evaluation of a ROX reference signal. The dye does not take part in the PCR reaction but allows to normalize for non-PCR related signal variation and provides a baseline in multiplex reactions. The dye is supplied as 100 μM concentration.
Content:
100 μM ROX dye in 20 mM Tris-HCl pH 8.5, 0.1 mM EDTA and 0.01 % Tween-20
Preparation of the qPCR master mix with ROX
Preparation of a master mix is crucial in quantitative PCR reactions to reduce pipetting errors. Vortex the ROX reference dye thoroughly before use.
| assay set-up | final ROX concentration |
| low ROX assay | 50 nM |
| high ROX assay | 500 nM |
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