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Proteinase K - Solid

from Tritirachium album

Endopeptidase K

Cat. No. Amount Price (EUR) Buy / Note
EN-181-1G 1 g 569,90 Add to Basket/Quote Add to Notepad

For general laboratory use.

Unit Definition: One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from haemoglobin as 1 μmol of tyrosine.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 6 months

Molecular Weight: 28.9 kDa

CAS#: 39450-01-6

EC number: 254-457-8

Purity: free of RNases, DNases and Exonucleases

Form: powder

Color: white

Specific activity: > 30 units/mg
DNase activity: not detectable
RNase activity: not detectable

Digestion of proteins during DNA and RNA preparation.

Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4 - 12.5).
Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
In presence of 0.5 - 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.

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