recombinant from Tritirachium album
Endopeptidase K
| Cat. No. | Amount | Price (EUR) | Buy / Note |
|---|---|---|---|
| EN-181-1G | 1 g | 284,80 | Add to Basket/Quote Add to Notepad |
| EN-181-10G | 10 x 1 g | 2.278,00 | Add to Basket/Quote Add to Notepad |
For general laboratory use.
Unit Definition: One unit is defined as the amount of enzyme required to hydrolyze casein to produce 1 μmol tyrosine per minute at 37°C and pH 8.0.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
Shelf Life: 12 months
Molecular Weight: 28.9 kDa
CAS#: 39450-01-6
EC number: 254-457-8
Purity: free of RNase, DNases and Nickase
Form: amorphous powder
Color: white to off white
Activity: > 30 units/mg
Applications:
Digestion of proteins during DNA and RNA preparation.
Description:
Proteinase K is a serine protease that exhibits a very broad cleavage specificity. The protein with a molecular weight of 28.9 kDa cleaves peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids. Proteinse K is not inactivated by metal chelating reagents such as EDTA or detergents such as SDS and is active over a wide range of pH (4 - 12.5).
Proteinase K is a highly active and stable protease with low cutting specificity. The enzyme belongs to the group of subtilisine-related serine proteases and is strongly inhibited by PMSF.
In presence of 0.5 - 1 % SDS Proteinase K inactivates DNases and RNases in eucaryotic and microbiological cell cultures. The use of Proteinase K during lysis of the cells allows the isolation of intact highly-molecular nucleic acids.
Hazard pictograms:
Signal word: Danger
Hazard statements:
H334 May cause allergy or asthma symptoms or breathing difficulties if inhaled.
Precautionary statements:
P304 + P340 IF INHALED: Remove person to fresh air and keep comfortable for breathing.
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