TAE Buffer - 10x conc.
For research use only.
Please centrifuge briefly before opening (volume ≤2 ml).
Description:
A 10 x concentration of TAE (Tris-Acetate-EDTA) Buffer contains:
0.4 M Tris
0.01 M EDTA
0.2 M Acetic Acid
TAE is an extensivley used buffer for agarose gel electrophoresis applications requiring high resolution and separation of high molecular weight, double-stranded DNA. TAE Buffer is more compatible with in-gel manipulations and band recovery procedures than TBE Buffer.
Shipping: shipped at ambient temperature
Storage Conditions: store at 4 °C
Shelf Life: 12 months
Form: liquid
Concentration: 10x
pH: 8.2 - 8.4 (22 °C)
Quality:
- Molecular biology grade
- sterile filtered, filter pore size 0.22 μm
- DNase / RNase free
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Scientific citations for Jena Bioscience bu-119-10
4 scientific citations and 2 citation images found by Bioz.
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Structural insights into the mechanism of GTP initiation of microtubule assembly.
Nature Communications, 2023-09-27 · PMID:37749104
To assemble S2-GTPγS-MTs, S2-tubulin (4mg/ml in stock) was reconstituted at
1.5–2mg/ml inBRB80 buffer (80mM 1,4-piperazinediethanesulfonic acid [PIPES],pH 6.9 , 1mM ethylene glycol tetraacetic acid [EGTA], 1mM MgCl2) supplemented with 1.5mM GTPγS (Jena Bioscience , Germany), 1mM DTT and 5% DMSO (vol/vol).. Themixturewas then incubated at 28 °C for 2–4h for S2-GTPγS-MTs .. -
Design, Synthesis, and Evaluation of Peptides Derived from L1 Protein Against Bovine Papillomavirus-1/2 Identified Along Mexico’s Cattle Export Route
Journal of Veterinary Research, 2023-03-01 · PMID:37008764
.. for 1 min at 60°C and final extension for 5 min at 72°C. ... Electrophoresis was performed with
tris acetate ethylenediaminetetraacetic acid buffer at 70 V for 30 min with a 100-bp molecular weight marker (Jena Bioscience , Jena, Germany).. The PCR products were purified with an Agarose Gel Extraction Kit (cat. no. PP-202S; .. -
Molecular diagnosis of inversions resulting from intron 22 rearrangement of F8 gene in a patients with hemophilia A and their families and genotype-phenotype association analysis
Europe PMC, 2022-07-22 · DOI:10.21203/rs.3.rs-1258088
In the nal step, PCR was carried out in each reaction containing 10μl of circularized DNA, in the presence of 0/6 μM of each primer (100 pmol/ μl) (Bioneer, South Korea), 0/5 U of Taq DNA Polymerase (Jena Bioscience, Germany) and PCR reagents, including;
buffer (10X) (Jena Bioscience , Germany), MgCl2 (25 mM) (Jena Bioscience, Germany) and dNTP (10 mM) (Jena Bioscience, Germany) in a total volume of 25 μl for the PCR reaction.
