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XP Up Screen

Crystallization Screen for high TEW concentrations

Cat. No. Amount Price (EUR) Buy / Note
CS-351 1 Kit 617,00 Add to Basket/Quote Add to Notepad

Shipping: shipped at ambient temperature

Storage Conditions: store at 8-10 °C

Shelf Life: 12 months

Description:
The additive TEW has proven to promote crystal contacts, improve crystal quality and further provides a valuable anomalous signal for phasing due to 6 tungsten atoms[1]. It has successfully induced protein crystallization at low concentrations such as 1 mM[2,3]. However, own experiments revealed that a higher concentration of 5 or 10 mM TEW can significantly increase crystallizability once again.
The XP Up Screen provides 96 of the most prominent crystallization conditions that have proven to be successfully upgraded with up to 10 mM TEW. To offer full flexibility in terms of final concentration, TEW is provided in an extra tube to be added to the protein droplet only. The 96 screening solutions are long-term stable also in the presence of up to 10 mM TEW allowing maximum flexibility with no compromise.

Content:
96 screening solutions optimized for high TEW concentrations (1,7 ml each)
5 mg TEW (1x X-TEW-5), dissolve in 23 μl water to achieve a 100 mM stock solution

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Selected References:
[1] Bijelic et al. (2017) Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography. Acc. Chem. Res. 50:1441.
[2] Sobala et al. (2020) Structure of human endo-α-1,2-mannosidase (MANEA), an antiviral host-glycosylation target. PNAS 117 (47):29595.
[3] Ames et al. (2020) Identifying a Molecular Mechanism That Imparts Species-Specific Toxicity to YoeB Toxins. Front Microbiol 11:959.