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The racing car among Bst Polymerases

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DNA amplification at constant temperature (Isothermal Amplification) offers key advantages over conventional PCR. Not only cost efficiency and simplicity are arguments for strand displacement techniques, also a high level of tolerance towards most PCR inhibitors makes this technology interesting for diagnostics, e.g. point-of-care applications worldwide.

Here we introduce a new innovative polymerase to reduce typical amplification times by a factor of 2-3 (Fig. 1). Saphir Bst2.0 Turbo benefits from an additional DNA-binding domain to ensure ultra-fast and robust amplification at 60 - 65 °C. Detect your target gene in just 5-10 min! Further, Saphir Bst2.0 Turbo has been developed to tolerate high salt concentrations for efficient direct analysis of blood samples or other difficult templates in diagnostics.

Figure 1: Rapid isothermal amplification of  target DNA (100 pg, 10 pg, 1 pg template) with Saphir Bst2.0 Turbo (blue; #PCR-390) compared to normal Saphir Bst2.0 (red, #PCR-389), Incubation at 65°C

Figure 1: Rapid isothermal amplification of target DNA (100 pg, 10 pg, 1 pg template) with Saphir Bst2.0 Turbo (blue; #PCR-390) compared to normal Saphir Bst2.0 (red, #PCR-389), Incubation at 65 °C.

Product Cat. No. Amount Price
Bst Polymerase
Saphir Bst2.0 Turbo Polymerase PCR-390S 2.000 units 118,50 €
PCR-390L 10.000 units 474,00 €
Bst Master Mixes
Saphir Bst2.0 Turbo GreenMaster PCR-393S 2x 1,25 ml 128,25 €
PCR-393L 10x 1,25 ml 513,00 €
Saphir Bst2.0 Turbo GreenMaster highROX PCR-394S 2x 1,25 ml 128,25 €
PCR-394L 10x 1,25 ml 513,00 €

Questions or inquiries?

Dr. Buerk Schaefer

All master mixes are also available as lyophilisates!

Get in touch for further information at pcr@jenabioscience.com or meet us at Medlab in Dubai (February 03 - 06, 2020; booth number Z6.F35).