Abberior® STAR 580 and STAR RED dyes are hydrophilic fluorescent dyes with remarkable photostability that give optimal performance of dye-conjugates in STED and confocal microscopy.
Jena Bioscience now offers STAR 580- and STAR RED-labeled single nucleotides as well as the corresponding HighYield T7 RNA Labeling Kits for in vitro transcription-based direct labeling of RNA probes.
Linker-optimized abberior® STAR dye-labeled UTPs in combination with an optimized enzyme mix allow efficient preparation of abberior® STAR dye-labeled RNA probes (> 100 nt up to several thousand nt) (Fig. 1, Tab. 1).
Product | Dye | Substitute for | Excmax / Emmax [nm] |
Recommended range of substitution |
---|---|---|---|---|
UTP-PEG5-STAR 580 | abberior® STAR 580 | AF555 (Alexa Fluor®555) / Cy3 / AF 594 Alexa Fluor® 594) | 587 / 607 | 30 - 50 % |
HighYield T7 STAR 580 RNA Labeling Kit | ||||
UTP-PEG5-STAR RED | abberior® STAR RED | AF647 (Alexa Fluor®647), Cy5 | 638 / 655 | 20 - 40 % |
HighYield T7 STAR RED RNA Labeling Kit |
Figure 1: In vitro transcription-based direct labeling of RNA probes with abberior® STAR dye-labeled UTPs. A) Comparison of UTP-PEG5-STAR 580 & UTP-PEG5-AF555. B) Comparison of UTP-PEG5-STAR RED & UTP-PEG5-AF647.
1.4 kbp RNA was generated using HighYield T7 AFXXX RNA Labeling Kit components in combination with an abberior® STAR or AF (Alexa Fluor®) - labeled UTP. Reaction conditions: 1 µg DNA template, 30 min at 37 °C. Crude in vitro reaction mix was subsequently analyzed be agarose gel electrophoresis (Visualization of total RNA with RNA intercalator EtBr / 0 – 100 %: Increasing percentage of UTP substitution by labeled UTP).
Contact us if you are interested in different abberior® dye-labeled nucleotides!