Abberior® STAR 580 and STAR RED dyes are hydrophilic fluorescent dyes with remarkable photostability that give optimal performance of dye-conjugates in STED and confocal microscopy.
Jena Bioscience now offers STAR RED-labeled UTP as well as the corresponding HighYield T7 RNA Labeling Kit for in vitro transcription-based direct labeling of RNA probes.
Linker-optimized abberior® STAR dye-labeled UTPs in combination with an optimized enzyme mix allow efficient preparation of abberior® STAR dye-labeled RNA probes (> 100 nt up to several thousand nt) (Fig. 1, Tab. 1).
| Product | Dye | Substitute for | Excmax / Emmax [nm] |
Recommended range of substitution |
|---|---|---|---|---|
| UTP-PEG5-STAR RED | abberior® STAR RED | AZDye 647 / Alexa Fluor®647 / Cy5 | 638 / 655 | 20 - 40 % |
| HighYield T7 STAR RED RNA Labeling Kit |
Figure 1: In vitro transcription-based direct labeling of RNA probes with abberior® STAR dye-labeled UTP. Comparison of UTP-PEG5-STAR RED & UTP-PEG5-AZDye 647.
1.4 kbp RNA was generated using HighYield T7 AZDye 647 RNA Labeling Kit components in combination with an abberior® STAR or AZDye-labeled UTP. Reaction conditions: 1 µg DNA template, 30 min at 37 °C. Crude in vitro reaction mix was subsequently analyzed be agarose gel electrophoresis (Visualization of total RNA with RNA intercalator EtBr / 0 – 100 %: Increasing percentage of UTP substitution by labeled UTP).
Contact us if you are interested in different abberior® dye-labeled nucleotides!
