LEXSY (Leishmania Expression System) is a eukaryotic protein expression platform based on the S1-classified protozoan L. tarentolae. It combines the advantages of prokaryotic systems (simple handling, fast growth) with those of eukaryotic ones (efficient folding, post-translational modifications).
More than 20 crystal structures of proteins produced with LEXSY have been reported so far, among them human Cu/Zn Superoxide dismutase, Channelrhodopsin 2 or Legumain.
Here LEXSY has been successfully employed by Ebner et al. to express an active dimeric chitinase from the gastrointestinal nematode Trichuris suis (Pig whipworm), which was suitable for in vitro and in vivo assays as well as for crystallization (Fig. 1).
 Gazdag et al. (2010) Purification and crystallization of human Cu/Zn superoxide dismutase recombinantly produced in the protozoan Leishmania tarentolae. Acta Crystallogr. Sect. F. Struct. Biol. Cryst. Commun. 66:871.
 Volkov et al. (2017) Structural insights into ion conduction by channelrhodopsin 2. Science 358, eaan8862.
 Zauner et al. (2018) Crystal Structure of Plant Legumain Reveals a Unique Two-Chain State with pH-Dependent Activity Regulation. Plant Cell 30:686.
 Ebner et al. (2021) A Helminth-Derived Chitinase Structurally Similar to Mammalian Chitinase Displays Immunomodulatory Properties in Inflammatory Lung Disease. J. Immunol. Res., Article ID 6234836.