LEXSY (Leishmania Expression System) is a eukaryotic protein expression platform based on the S1-classified protozoan L. tarentolae. It combines the advantages of prokaryotic systems (simple handling, fast growth) with those of eukaryotic ones (efficient folding, post-translational modifications).
Antigens of different groups of pathogens (viruses, bacteria, protozoa), ranging from proteins to complex virus-like particles (Figure 1), have been successfully expressed with LEXSY and were used for e.g., structure determination and immunization experiments (Table 1).
|Hendravirus attachment protein||Fischer et al. (2016)|
|Trypanosoma brucei antigens||Ferrer et al. (2020)|
|Hepatitis B virus like particles||Czarnota et al. (2020)|
|Hepatitis B small surface antigen||Czarnota et al. (2016)|
|Hepatitis C virus like particles||Bolhassani et al. (2017)|
|HPV16 L1 virus like particles||Bolhassani et al. (2015)|
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 Fischer et al. (2016) Expression, characterisation and antigenicity of a truncated Hendra virus attachment protein expressed in the protozoan host Leishmania tarentolae. J. Virol. Methods 228:48.
 Ferrer et al. (2019) Production of Recombinant Trypanosoma cruzi Antigens in Leishmania tarentolae. in 105–118. doi:10.1007/978-1-4939-9148-8_8
 Czarnota et al. (2020) Specific antibodies induced by immunization with hepatitis B virus-like particles carrying hepatitis C virus envelope glycoprotein 2 epitopes show differential neutralization efficiency. Vaccines 8:1.
 Czarnota et al. (2016) Immunogenicity of Leishmania-derived hepatitis B small surface antigen particles exposing highly conserved E2 epitope of hepatitis C virus. Microb. Cell Fact. 15:62.
 Bolhassani et al. (2017) Comparison of HCV Core and CoreE1E2 Virus-Like Particles Generated by Stably Transfected Leishmania tarentolae for the Stimulation of Th1 Immune Responses in Mice. Curr. Drug Deliv. 14:1040.
 Bolhassani et al. (2015) VLP production in Leishmania tarentolae: A novel expression system for purification and assembly of HPV16 L1. Protein Expr. Purif. 116:7.