Complex proteins are often poorly expressed in prokaryotic expression systems. With LEXSY you can tackle these difficult proteins with a eukaryotic host – but with all the ease and simplicity known from prokaryotic cells.
LEXSY (Leishmania Expression System) is a eukaryotic protein expression platform based on the S1-classified protozoan L. tarentolae. It combines the advantages of prokaryotic systems (simple handling, fast growth) with those of eukaryotic ones (efficient folding, posttranslational modifications).
Of more than 100 proteins, whose expression in other systems like E. coli, yeast, insect or mammalian cells was unsatisfactory, more than 90 % were successfully expressed in soluble and active form with LEXSY (Figure 1, Table 1).
|Protein||Origin||Problems with other systems||Reference|
|Hydroxynitrile Lyase (MeHNL)||Cassava plant||insoluble||Dadashipour et al.|
|Laminin-332||human||non-homogenous product||Phan et al.|
|N-Acetyl Serotonin Methyl Transferase (ASMT)||human||degradation||Ben-Abdallah et al.|
|Proprotein Convertase 4||rat||low yield||Basak et al.|
|Tissue Plasminogen Activator||human||not folded, hyperglycosylation||Hemayatkar et al.|
|Sodium-nucleobase transporter rSNBT1||human||low yield, improper targeting||Doukas et al.|
 Dadashipour et al. (2011) Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems. Protein Expr. Purif. 77:92.
 Phan et al. (2009) The production of recombinant human laminin-332 in a Leishmania tarentolae expression system. Protein Expr. Purif. 68:79.
 Ben-Abdallah et al. (2011) Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae. Protein Expr. Purif. 75:114.
 Basak et al. (2008) Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: Purification, biochemical study and inhibitor design. Protein Expr. Purif. 60:117.
 Hemayatkar et al. (2010) Increased expression of recombinant human tissue plasminogen activator in Leishmania tarentolae. Biotechnol. J. 5:1198.
 Doukas et al. (2019) Heterologous expression of the mammalian sodium-nucleobase transporter rSNBT1 in Leishmania tarentolae. Biochim. Biophys. acta. Biomembr. 1861:1546.