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LEXSY for difficult-to-express proteins

Complex proteins are often poorly expressed in prokaryotic expression systems. With LEXSY you can tackle these difficult proteins with a eukaryotic host – but with all the ease and simplicity known from prokaryotic cells.

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LEXSY (Leishmania Expression System) is a eukaryotic protein expression platform based on the S1-classified protozoan L. tarentolae. It combines the advantages of prokaryotic systems (simple handling, fast growth) with those of eukaryotic ones (efficient folding, posttranslational modifications).

Of more than 100 proteins, whose expression in other systems like E. coli, yeast, insect or mammalian cells was unsatisfactory, more than 90 % were successfully expressed in soluble and active form with LEXSY (Figure 1, Table 1).

Figure 1: Success rates of LEXSY protein expression...
Figure 1: Success rates of LEXSY protein expression: Over 100 proteins have been expressed in LEXSY to date. An overall success rate of more than 90 % makes LEXSY the expression system of choice for difficult to express proteins.

Table 1: Selection of proteins successfully expressed with LEXSY, where expression with other systems failed:

Protein Origin Problems with other systems Reference
Hydroxynitrile Lyase (MeHNL) Cassava plant insoluble Dadashipour et al.[1]
Laminin-332 human non-homogenous product Phan et al.[2]
N-Acetyl Serotonin Methyl Transferase (ASMT) human degradation Ben-Abdallah et al.[3]
Proprotein Convertase 4 rat low yield Basak et al.[4]
Tissue Plasminogen Activator human not folded, hyperglycosylation Hemayatkar et al.[5]
Sodium-nucleobase transporter rSNBT1 human low yield, improper targeting Doukas et al.[6]

Need further information?

Dr. Andreas Licht

Please check out the LEXSY section or contact us by email at expression@jenabioscience.com !


[1] Dadashipour et al. (2011) Comparative expression of wild-type and highly soluble mutant His103Leu of hydroxynitrile lyase from Manihot esculenta in prokaryotic and eukaryotic expression systems. Protein Expr. Purif. 77:92.
[2] Phan et al. (2009) The production of recombinant human laminin-332 in a Leishmania tarentolae expression system. Protein Expr. Purif. 68:79.
[3] Ben-Abdallah et al. (2011) Production of soluble, active acetyl serotonin methyl transferase in Leishmania tarentolae. Protein Expr. Purif. 75:114.
[4] Basak et al. (2008) Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: Purification, biochemical study and inhibitor design. Protein Expr. Purif. 60:117.
[5] Hemayatkar et al. (2010) Increased expression of recombinant human tissue plasminogen activator in Leishmania tarentolae. Biotechnol. J. 5:1198.
[6] Doukas et al. (2019) Heterologous expression of the mammalian sodium-nucleobase transporter rSNBT1 in Leishmania tarentolae. Biochim. Biophys. acta. Biomembr. 1861:1546.