Recombinantly produced antigens are essential for basic research, diagnosis and as vaccines, and their correct glycosylation pattern is pivotal for proper antigen recognition. Murphy et al. recently showed that only Trypanosoma cruzi TcI antigen produced with LEXSY is usable in serodiagnosis[1], whereas antigen produced in E. coli or synthetic peptides failed to react with serum.
The reason was found to be glycosylation: LEXSY is able to recognize glycosylation sites and provides recombinantly expressed proteins with correct and homogenous N- and O-glycosylation patterns (Figure 1)[2,3].
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