Double-stranded RNA (dsRNA) is not only a hallmark of viral infections but also a key molecule in RNA interference (RNAi)-mediated gene silencing at the mRNA level.
Large amounts of >200 bp dsRNA (> 2 mg/ml transcription reaction) are efficiently produced using the HighYield T7 RNAi Kit via T7 RNA Polymerase-mediated in vitro transcription (Fig. 1). The resulting dsRNA can subsequently be used for RNA interference (RNAi) applications by direct administration e.g. on plant tissues or as template for enzymatic digestion to short interfering RNA (siRNA)[2-4].
Figure 1: Similar results with Jena Bioscience’s HighYield T7 RNAi Kit and the T7 RiboMAX™ Express RNAi System.
A) dsRNA is synthesized in a 3 step procedure.
B) Similar transcription efficiency of Jena Bioscience’s HighYield T7 RNAi Kit and T7 RiboMAX™ Express RNAi System. In vitro transcription has been performed according to the manufacturer protocol using 1 µg DNA template 1. Transcription efficiency was analyzed by agarose gelelectrophoresis (equal volumes of in vitro transcription reactions). JBS: HighYield T7 RNAi Kit, Promega: T7 RiboMAX™ Express RNAi System
C) Competitive pricing of Jena Bioscience’s HighYield T7 RNAi Kit.
Please contact Barbara with all questions or inquiries you may have!
 Voloudakis et al. (2015) Efficient Double-Stranded RNA Production Methods for Utilization in Plant Virus Control. Plant Virology Protocols. Methods in Molecular Biology 1236:1.
 Sun et al. (2018) A Simple and Cost-Effective Approach for in vitro production of sliced siRNAs as potent triggers for RNAi. Molecular Therapy Nucleic Acids 8:345.
 Kittler et al. (2005) Production of endoribonuclease-prepared short interfering RNAs for gene silencing in mammalian cells. Nature Methods 2 (10): 779.
 Buchholz et al. (2006) Enzymatically prepared RNAi libraries. Nature Methods 3 (9):696.