Structural investigations by NMR or X-ray crystallography require a homogenous RNA preparation. However, in vitro transcription-based RNA synthesis with T7 RNA polymerase produces heterogenous RNA at both 5' and 3' ends especially if prepared under the commonly used class III G-initiating phi6.5 promotor.
The combination of
|Product||Cat.-No.||Amount||Price||Application||Average yield / react.*|
|HighYield T7 P&L RNA Synthesis Kit||RNT-201||50 react.||195,00 €||Optimized kit for high yield RNA synthesis||140 - 160 µg|
|T7 P&L RNA Polymerase HC||RNT-018||50 µl (2 µg/µl)||365,00 €||Single enzyme for individual assay set-ups||n/a|
|HighYield T7 P&L RNA NMR Kit (5F-UTP)||RNT-202-S||15 react.||395,00 €||Optimized kit for preparation of 5-Fluoro-modified RNA||80 µg|
|RNT-202-L||50 react.||895,00 €|
|5-Fluoro-UTP (5F-UTP)||NU-1115S||10 µl (100 mM)||123,24 €||Single nucleotide for individual assay set-up||n/a|
|NU-1115L||5x 10 µl (100 mM)||360,95 €|
|HighYield T7 P&L RNA NMR Kit (2F-ATP)||RNT-203-S||15 react.||535,00 €||Optimized kit for preparation of 2-Fluoro-modified RNA||80 µg|
|RNT-203-L||50 react.||1.140,00 €|
|2-Fluoro-ATP (2F-ATP)||NU-145S||10 µl (100 mM)||180,47 €||Single nucleotide for individual assay set-up||n/a|
|NU-145L||5x 10 µl (100 mM)||523,36 €|
Please contact Barbara with all questions or inquiries you may have!
 Coleman et al. (2004) Superior 5‘ homogeneity of RNA from ATP-initiated transcription under T7 phi 2.5 promoter. Nucleic Acids Research 32 (1):e14.
 Guillerez et al. (2005) A mutation in T7 RNA polymerase that facilitates promoter clearance. Natl. Acad. Sci. U.S.A. 102:5958.
 Salvail-Lacoste et al. (2018) Affinity purification of T7 RNA transcripts with homogeneous ends using ARiBo and CRISPR tags. RNA 19:1003.