FORMOscreen®: The best FDA/EMA-approved formulations for your antibody

The FORMOscreen® is designed to quickly and simply identify the most suitable buffer for any antibody. The screen contains 96 carefully selected buffers from FDA/EMA-approved formulations of therapeutic antibodies. It comes in a deep well block with 230 µl of a 5-fold stock of each buffer and thus allows for screening with high-throughput methods such as DSF and nanoDSF, or with any other method of choice, e.g. DSC, DLS, LC-MS, SEC-HPLC or ELISA.

Depending on the chosen method, the FORMOscreen® provides important buffer-dependence data for antibody aggregation propensity, chemical and thermal stability, long-term storage stability, forced-degradation resistance, as well as biochemical activity and antigen-binding.

In addition, the FORMOscreen® works also great with non-antibody proteins and can tell important information on their stability, aggregation, and activity behavior in different buffers.

Design and Features

96 buffer conditions from FDA/EMA-approved therapeutic antibody formulations
Ready-to-use 5-fold buffer stocks, 230 µl each
Covers the most widely applied formulation range of pH, buffer substances/concentrations, ionic strength, sugars, amino acids and detergents

Advantages

All buffer conditions have been approved for use in antibody formulations by regulatory authorities
All buffer conditions have already proven to be beneficial for stability and integrity of antibodies and their derivatives in final formulations
Ready-to-use, concentrated buffer stock format enables application with almost any analytical method and at almost any protein concentration
Works also great with non-antibody proteins

Application example

NanoDSF thermal melting of two antibodies in the 96 FORMOscreen® conditions result in melting temperatures between 46.7 and 68.4 °C for mAb 1 and 57.2 and 80.4 °C for mAb 2. The upper panels show the raw fluorescence signal (350/330 nm ratio); the lower panels show the first derivative curves^[1].

Questions or inquiries?

E-mail Christin for technical inquiries or further information: xtals@jenabioscience.com

Reference:

[1] Schmid et al. (2020) FORMOscreen®: Buffers of Formulations of Developed Antibodies for Pre-Formulation of Biologics. 2bind GmbH application note.