The FORMOscreen® is designed to quickly and simply identify the most suitable buffer for any antibody. The screen contains 96 carefully selected buffers from FDA/EMA-approved formulations of therapeutic antibodies. It comes in a deep well block with 230 µl of a 5-fold stock of each buffer and thus allows for screening with high-throughput methods such as DSF and nanoDSF, or with any other method of choice, e.g. DSC, DLS, LC-MS, SEC-HPLC or ELISA.
Depending on the chosen method, the FORMOscreen® provides important buffer-dependence data for antibody aggregation propensity, chemical and thermal stability, long-term storage stability, forced-degradation resistance, as well as biochemical activity and antigen-binding.
In addition, the FORMOscreen® works also great with non-antibody proteins and can tell important information on their stability, aggregation, and activity behavior in different buffers.
NanoDSF thermal melting of two antibodies in the 96 FORMOscreen® conditions result in melting temperatures between 46.7 and 68.4 °C for mAb 1 and 57.2 and 80.4 °C for mAb 2. The upper panels show the raw fluorescence signal (350/330 nm ratio); the lower panels show the first derivative curves.
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