The Leishmania tarentolae based LEXSY in vitro expression system offers protein yields of up to 300 µg/ml of reaction volume and is with € 2.50 per reaction the most economic system on the market.
LEXSY in vitro is well suited for expressing membrane proteins and expresses large proteins reliably: All six subunits of the HOPS and CORVET membrane tethering complexes – in total 600 kDa – have been expressed and successfully reconstituted using LEXSY in vitro (Fig. 1).
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 Ruehrer et al. (2013) Exploiting Leishmania tarentolae cell-free extracts for the synthesis of human solute carriers. Mol. Membr. Biol. 30:288.
 Guo et al. (2013) Subunit organisation of in vitro reconstituted HOPS and CORVET multisubunit membrane tethering complexes. PLoS One 8:e81534.