Double-stranded (ds)RNA formation is a hallmark of viral infections that is essential for the induction of innate immunity.
Anti-dsRNA monoclonal antibodies are efficient tools for the detection of dsRNA in cell culture and tissues such as FFPE samples1-6 (Fig. 1, Tab. 1) e.g.
Figure 1: A) Immunofluorescence detection of dsRNA in SARS-CoV infected Vero cells (3) with Anti-dsRNA monoclonal antibody J2. 1: uninfected cells. Bar: 20 µM (modified according to [4]). B) Immune electron microscopy of dsRNA in double-membrane vesicles (DMVs) of SARS-Cov infected Vero E6 cells with Anti-dsRNA monoclonal antibody J2 (modified according to [5]). C) dsRNA detection in FFPE tissues of Coxsackie virus infected neonatal mouse with Anti-dsRNA monoclonal antibody J2. 1: mouse heart, 2: brown fat, 3: pancreas, 4: salivary gland, 5: CNS ganglia, 6: uninfected tissue (modified according to [6]).
| Product | Description | Application | Selection guide |
|---|---|---|---|
| Anti-dsRNA monoclonal antibody J2 | Mouse, IgG2a, kappa light chain | ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy | Gold standard for dsRNA detection |
| Anti-dsRNA monoclonal antibody K1 | Mouse, IgG2a, kappa light chain | Recommended for Poly I:C detection J2 alternative in case of cross reactions |
|
| Anti-dsRNA monoclonal antibody K2 | Mouse, IgM, kappa light chain | ELISA, IHC, Dot Blot | Isotype alternative to J2 & K1 Recommended for (Sandwich-) ELISA |
Please contact Barbara with all questions or inquiries you may have!
[1] Schönborn et al. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res. 19:2993.
[2] Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47:255.
[3] Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford.
[4] Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80 (10):5059.
[5] Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6 (9):e226.
[6] Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 80.
