» Sign in / Register

The gold standard in viral dsRNA detection: Monoclonal J2, K1 & K2 antibodies

visit product page

Double-stranded (ds)RNA formation is a hallmark of viral infections that is essential for the induction of innate immunity.

Anti-dsRNA monoclonal antibodies are efficient tools for the detection of dsRNA in cell culture and tissues such as FFPE samples1-6 (Fig. 1, Tab. 1) e.g.

  • for characterization & detection of viruses with dsRNA genomes or intermediates (including SARS, Hepatitis C, Dengue or West Nile Virus).
  • as diagnostic tool for determination whether an unknown pathogen is of viral or bacterial origin.

Figure 1: A) Immunofluorescence detection of dsRNA in SARS-CoV infected Vero cells (3) with SCICONS Anti-dsRNA monoclonal antibody J2.

Figure 1: A) Immunofluorescence detection of dsRNA in SARS-CoV infected Vero cells (3) with Anti-dsRNA monoclonal antibody J2. 1: uninfected cells. Bar: 20 µM (modified according to [4]). B) Immune electron microscopy of dsRNA in double-membrane vesicles (DMVs) of SARS-Cov infected Vero E6 cells with Anti-dsRNA monoclonal antibody J2 (modified according to [5]). C) dsRNA detection in FFPE tissues of Coxsackie virus infected neonatal mouse with Anti-dsRNA monoclonal antibody J2. 1: mouse heart, 2: brown fat, 3: pancreas, 4: salivary gland, 5: CNS ganglia, 6: uninfected tissue (modified according to [6]).

Table 1: Overview on dsRNA detection products

Product Description Application Selection guide
Anti-dsRNA monoclonal antibody J2 Mouse, IgG2a, kappa light chain ELISA, IF, FACS, IHC, IP, Dot Blot, ChIP, affinity purification, immunoelectron microscopy Gold standard for dsRNA detection
Anti-dsRNA monoclonal antibody K1 Mouse, IgG2a, kappa light chain Recommended for Poly I:C detection
J2 alternative in case of cross reactions
Anti-dsRNA monoclonal antibody K2 Mouse, IgM, kappa light chain ELISA, IHC, Dot Blot Isotype alternative to J2 & K1
Recommended for (Sandwich-) ELISA

Dr. Barbara Zschoernig

Please contact Barbara with all questions or inquiries you may have!

Selected References:

[1] Schönborn et al. (1991) Monoclonal antibodies to double-stranded RNA as probes of RNA structure in crude nucleic acid extracts. Nucleic Acids Res. 19:2993.
[2] Lukacs (1994) Detection of virus infection in plants and differentiation between coexisting viruses by monoclonal antibodies to double-stranded RNA. J. Virol. Methods 47:255.
[3] Lukacs (1997) Detection of sense:antisense duplexes by structure-specific anti-RNA antibodies. In: Antisense Technology. A Practical Approach, C. Lichtenstein and W. Nellen (eds), pp. 281-295. IRL Press, Oxford.
[4] Weber et al. (2006) Double-Stranded RNA is produced by positive-strand RNA viruses and DNA viruses but not in detectable amounts by negative-strand RNA viruses. Journal of Virology 80 (10):5059.
[5] Knoops et al. (2008) SARS-Coronavirus replication is supported by a reticulovesicular network of modified endoplasmic reticulum. PLOS Biology 6 (9):e226.
[6] Richardson et al. (2010) Use of antisera directed against dsRNA to detect viral infections in formalin-fixed paraffin-embedded tissue. Journal of Clinical Virology 49: 80.