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Nick Translation-based DNA Probe Labeling

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Labeling of DNA probes for in situ hybridization is most often performed by enzymatic incorporation of labeled dUTP using a mixture of DNase I / DNA polymerase I (Nick Translation) (Tab. 1) (Ribgy et al. (1977), Morrison et al. (2003), Wiegant et al. (2001)).

Check out our ready-to use Fluorescent, Biotin and Digoxigenin Nick Translation Labeling Kits!

Figure 1: Metaphase plates of an Arowana fish highlighting the location of 18S rRNA genes after their labeling with ATTO488 NT Labeling Kit through Fluorescence in situ (FISH) experiments.

Figure 1: Metaphase plates of an Arowana fish highlighting the location of 18S rRNA genes after their labeling with ATTO488 NT Labeling Kit through Fluorescence in situ (FISH) experiments. A. DAPI staining. B. 18S rRNA signals C. merged image (courtesy of Prof. Marcelo de Bello Cioffi, Federal University of São Carlos).

 

Table 1: Overview on available Nick Translation (NT) Kits (* Alexa Fluor (AF))

Kit Label Labeling principle similar to... Selected References
Hapten Nick Translation Kits
Biotin 16 NT Labeling Kit Biotin
(via Biotin-16-dUTP)
Biotin-Nick Translation Mix
(Roche)
Matsuyama et al. (2017)
Malinovskaya et al. (2018)
Biotin 14 NT Labeling Kit
NEW
Biotin
(via Biotin-14-dATP)
Bio Nick DNA Labeling System
(Thermo Scientific)
Digoxigenin NT Labeling Kit Digoxigenin
(via Digoxigenin-11-dUTP)
DIG-Nick Translation Mix
(Roche)
Fluorescent Nick Translation Kits
ATTO425 NT Labeling Kit ATTO425
(via dUTP-ATTO-425
Chatre et al. (2015)
Fluorescein NT Labeling Kit Fluorescein
(via Fluorescein-12-dUTP)
BioProbe Nick Translation Kit with Fluorescein-12-dUTP
(Enzo Life Science)
ATTO488 NT Labeling Kit ATTO488
(via dUTP-XX-ATTO-488)
Himmelbach et al. (2018)
Kwiatek et al. (2017)
Chatre et al. (2015)
AF*488 NT Labeling Kit
NEW
AF*488
(via dUTP-XX-AF*488)
Cy3 NT Labeling Kit
NEW
Cy3
(via dUTP-XX-Cy3)
ATTO550 NT Labeling Kit ATTO550
(via dUTP-ATTO-550)
Himmelbach et al. (2018)
Kwiatek et al. (2017)
Mirzaghaderi et al. (2017)
Chatre et al. (2015)
TexasRed NT Labeling Kit Texas Red
(via dUTP-TexasRed)
AF*594 NT Labeling Kit
NEW
AF*594
(via dUTP-XX-AF*594)
Cy5 NT Labeling Kit
NEW
Cy5
(via dUTP-XX-Cy5)
ATTO647N NT Labeling Kit ATTO647N Kwiatek et al. (2017)
Chatre et al. (2015)
 
Dr. Barbara Zschoernig

Didn’t find the labeling kit you are looking for? Please contact me at
info@jenabioscience.com !

Selected References:

  • Chatre et al. (2015) mTRIP: An Imaging Tool to Investigate Mitochondrial DNA Dynamics in Physiology and Disease at the Single-Cell Resolution. In: Weissig V., Edeas M. (eds) Mitochondrial Medicine. Methods in Molecular Biology 1264, Humana Press, New York, NY.
  • Himmelbach et al. (2018) Discovery of multi-megabase polymorphic inversions by chromosome conformation capture sequencing in large-genome plant species. The Plant Journal doi:10.1111/tpj.14109.
  • Kwiatek et al. (2017) Adaption of the Pivotal-Differential Genome Pattern for the Induction of Intergenomic Chromosome Recombination in Hybrids of Synthetic Amphidiploids within Triticeae Tribe. Frontiers in Plant Science 8 (1300):1.
  • Malinovskaya et al. (2018) Copy Number of Human Ribosomal Genes With Aging: Unchanged Mean, but Narrowed Range and Decreased Variance in Elderly Group. Frontiers in Genetics 9 (206):1.
  • Matsuyama et al. (2017) Lengthening of high-yield production levels of monoclonal antibody-producing Chinese hamster ovary cell by downregulation of breast cancer 1. Journal of Bioscience and Bioengineering 123 (3):382.
  • Mirzaghaderi et al. (2017) Dynamic nucleolar activity in wheat x Aegilops hybrids: evidence of C-genome dominance. Plant Cell Reports 36 (8):1277.
  • Morrison et al. (2003) Labeling Fluorescence In Situ Hybridization Probes for Genomic Targets. Methods in Molecular Biology 204:21.
  • Rigby et al. (1977) Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113 (1):237.
  • Wiegant et al. (1997) Probe Labeling and Fluorescence In Situ Hybridization. In: Current Protocols in Cytometry 8.3.1, John Wiley & Sons Inc.