Traditional dyes such as fluorescein or TAMRA are often hampered by low photostability and/or low hydrophilicity. Both however are critical parameter for detection sensitivity and bioconjugate solubility, respectively.
AF-Dyes (also known as Alexa Fluor® dyes) are hydrophilic fluorescent dyes with remarkable photostability. We now offer both functionalized AF-Dyes and AF-Dye – nucleotide conjugates. CLICK AF-Dyes are routinely used for Click Chemistry – based labeling.
| CLICK AF-Dyes | AF-Dye - Nucleotide conjugates | ||||||||
| Dye | Substitute for |
Excmax [nm]** |
Emmax [nm]** |
εmax* | Azide | Picolyl-Azide | Alkyne | DBCO*** = ADIBO/DIBAC |
dUTP |
| AF488 (also known as Alexa Fluor®488) |
Cy2 FITC (Fluorescein) ATTO488 |
494 | 517 | 73 | ✔ | ✔ | ✔ | ✔ | ✔ |
| AF546 (also known as Alexa Fluor®546) |
TAMRA | 554 | 570 | 110 | ✔ | ✔ | ✔ | ✔ | - |
| AF555 (structural analog to Alexa Fluor®555) |
Cy3 ATTO550 |
555 | 572 | 155 | ✔ | ✔ | ✔ | ✔ | + |
| AF594 (also known as Alexa Fluor®594) |
Texas Red | 590 | 617 | 92 | ✔ | ✔ | ✔ | ✔ | ✔ |
| AF647 (structural analog to Alexa Fluor®647) |
Cy5 ATTO647N |
648 | 671 | 270 | ✔ | ✔ | ✔ | ✔ | + |
Can’t find the nucleotide-dye conjugate you are looking for? Just contact us!
