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Aptamers are short single-stranded DNA or RNA oligonucleotides (< 100 nt). They gained increasing importance in drug discovery due to their inherent ability to form defined three-dimensional structures that enables them to bind to various targets (e.g. proteins) with antibody-antigen-like affinity and specificity[1].
Aptamers with the highest binding affinity and specificity against a given target molecule are generated by an multi-step in vitro selection and enzymatic amplification process called "systematic evolution of ligands by exponential enrichment (SELEX) (for detailed information please refer to the references below[2,3]).
The main advantages of aptamers compared to antibodies are heat stability, the lack of immunogenicity and minimal interbatch variability. Their usage in diagnostics however, is often hampered by nuclease-mediated degradation.
Enzymatic incorporation of fluoro-, amino- or O-methyl- 2'-ribose-modified nucleotides (1) by generating an initially modified combinatorial library or (2) post-SELEX markedly improve the nuclease resistance of aptamers (Tab. 1) [3-12]. Furthermore, a number of modified nucleotides suitable for the selection of cross-linking capable aptamers (Photo-SELEX) are available as well (Tab. 1)[13-15].
n/a: not applicable
Nucleotide | Modification | DNA aptamer selection (DNA SELEX) | RNA aptamer selection (RNA SELEX) | |
---|---|---|---|---|
Ribose Moiety | 2'F-dUTP | Substitution of 2'-OH by fluor (F) | [5] | [6,7] |
2'F-dCTP | [5] | [6,7] | ||
2'F-dATP | [5] | [6] | ||
2'F-dGTP | [5] | |||
2'OMe-UTP | Modification of 2'-OH by a methyl group (CH3) | [8,9,10,11] | ||
2'OMe-CTP | [8,9,11] | |||
2'OMe-ATP | [8] | |||
2'OMe-GTP | [8] | |||
2'NH2-dUTP | Substitution of 2'-OH by an amino group (NH2) | [6,12] | ||
2'NH2-dCTP | [12] | |||
2'NH2-dATP | ||||
2'NH2-dGTP | ||||
LNA-ATP | LNA (Locked Nucleic Acid) with methylene bridge beetween 2'-O and 4'-C | [16] | ||
LNA-GTP | ||||
LNA-CTP | ||||
LNA-UTP | ||||
Base Moiety | 5Br-dUTP | Modification of C-5 by Bromine (Br) | [13] | n/a |
5I-UTP | Modification of C-5 by Iodine (I) | n/a | [14] | |
4-Thio-UTP | Substitution of 4-O by Sulfur (S) | n/a | [15] |
[1] Hermann et al. (2000) Adaptive recognition by nucleic acid aptamers. Science 287:820.
[2] Stoltenburg et al. (2007) SELEX-A (r)evolutionary method to generate high-affinity nucleic acids ligands. Biomolecular Engineering 24:381.
[3] Lauridsen et al. (2012) Enzymatic Recognition of 2'-Modified Ribonucleoside 5'-Triphosphates: Towards the Evolution of Versatile Aptamers. Chem. Bio. Chem. 13:19.
[4] Keefe et al. (2008) SELEX with modified nucleotides. Current Opinion in Chemical Biology 12:448.
[5] Ono et al. (1997) 24-Fluoro modified nucleic acids: polymerase-directed synthesis, properties and stability to analysis by matrix-assisted laser desorption/ionization mass spectrometry. Nucleic Acids Research 25 (22):4581.
[6] Aurup et al. (1992) 2'-Fluoro and 2-amino-2'-deoxynucleoside 5'-triphosphates as substrates for T7 RNA polymerase. Biochemistry 31 (40):9626.
[7] Adler et al. (1998) POST-SELEX Chemical Optimization of a trypanosome-specific RNA aptamer. Comb. Chem. High Throughput Screen. 11 (1):16.
[8] Burmeister et al. (2005) Direct In Vitro Selection of a 2'-O-Methyl Aptamer to VEGF. Chemistry & Biology 12:25.
[9] Burmeister et al. (2006) 2'-Deoxy Purine, 2'-O-Methyl Pyrimidine (dRmY) Aptamers as Candidate Therapeutics. OLIGONUCLEOTIDES 16:337.
[10] Padilla et al. (1999) Efficient synthesis of nucleic acids heavily modified with non-canonical ribose 2'-groups using a mutant T7 RNA Polymerase (RNAP). Nucleic Acids Res. 27 (6):156.
[11] Padilla et al. (2002) A Y639F/H784A T7 RNA polymerase double mutant displays superior properties for synthesizing RNAs with non-canonical NTPs. Nucleic Acids Res. 30 (24):e138.
[12] Lin et al. (1994) Modified RNA sequence pools for in vitro selection. Nucleic Acids Res. 22 (24):5229.
[13] Golden et al. (2000) Diagnostic potential of PhotoSELEX-evolved ssDNA aptamers. Journal of Biotechnology 81:167.
[14] Jensen et al. (1995) Using in vitro selection to direct the covalent attachment of human immunodeficiency virus type 1 Rev protein to high-affinity RNA ligands. Proc. Natl. Acad. Sci. USA 92:12220.
[15] Park et al. (2008) Higher-Order Association States of Cellular ERBB3 Probed with Photo-Cross-Linkable Aptamers. Biochemistry 47 (46):11992.
[16] Crouzier et al. (2012) Efficient reverse transcription using locked nucleic acid nucleotides towards the evolution of nuclease resistant RNA aptamers. PLoS One. 7 (4):e35990.