Lysozyme - Solution

Lysozyme crystals grown in the presence of 8 % w/v Sodium Chloride and 100 mM Sodium Acetate pH 4.2.

For general laboratory use.

Shipping: shipped at ambient temperature

Storage Conditions: store at 4 °C
do not freeze

Shelf Life: 12 months

Molecular Weight: 14.3 kDa (single chain)

CAS#: 12650-88-3

EC number: 235-747-3

Form: aqueous solution

Concentration: 20 mg/ml

pH: 3.5 (H₂O, 20 °C)

Solubility: 300 g/l in water

Activity: 50 kU/mg (Micrococcus luteus, FIP-Standard; pH 7.0; 25 °C)

Applications:
Lysozyme can be utilized as model protein in crystallization experiments and X-ray structure analysis.

Description:
Lysozyme is a glycosidase which hydrolyzes the ß 1,4 glycosidic bond between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in the bacterial peptidoglycan^[1].

Source: Chicken egg white

Usage:
Crystals can be grown using the sitting drop, hanging drop or microbatch method.
Following crystallization conditions are suggested for lysozyme crystal growth:

• 0.7 - 1.5 M Sodium Chloride, 100 mM Sodium Acetate pH 4.0 - 4.8^[2]
• 10 % w/v Sodium Chloride, 5% v/v Propanol^[2]
• 0.44 M Sodium Nitrate, 100 mM Sodium Acetate pH 4.6^[2]

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Selected References:
[1] Rye et al. (2000) Glycosidase mechanisms. Curr. Opin. Chem. Biol. 4:573.
[2] Gilliland et al. (2002) The Biological Macromolecule Crystallization Database: crystallization procedures and strategies. Acta Cryst. D 58:916.