LAMP – loop-mediated DNA amplification at constant temperature (Isothermal Amplification) offers key advantages over conventional PCR. Not only cost efficiency and simplicity (no thermocycler required!) are arguments for strand displacement techniques. Also a high level of tolerance towards a large range of PCR inhibitors that enables impure sample amplification and direct detection of target genes makes this future technology interesting for diagnostics, e.g. point-of-care applications worldwide.
Here we introduce a new innovative enzyme to reduce typical amplification times by a factor of 2 - 3. Saphir Bst2.0 Turbo Polymerase benefits from an additional DNA-binding domain to ensure ultra-fast and robust amplification at 60 - 65 °C. Detect your target gene in just 5 - 10 min!
Figure 1: Rapid isothermal amplification of target DNA (100 pg, 10 pg, 1 pg template) with Saphir Bst2.0 Turbo DNA Polymerase (blue; #PCR-390) compared to normal Saphir Bst2.0 Polymerase (red, #PCR-389). Incubation at 65 °C.
| Product | Cat. No. | Amount | Price |
|---|---|---|---|
| Bst Polymerase | |||
| Saphir Bst2.0 Turbo Polymerase | PCR-390S | 2000 Units | 147,20 € |
| PCR-390L | 5 x 2.000 Units | 588,40 € | |
| Saphir Bst2.0 Polymerase | PCR-389S | 2000 Units | 98,10 € |
| PCR-389L | 5 x 2.000 Units | 392,50 € | |
| Bst Master Mixes | |||
| Saphir Bst2.0 Turbo GreenMaster | PCR-393S | 2 x 1,25 ml | 159,20 € |
| PCR-393L | 10 x 1,25 ml | 636,90 € | |
| Saphir Bst2.0 GreenMaster | PCR-387S | 2 x 1,25 ml | 106,10 € |
| PCR-387L | 10 x 1,25 ml | 424,60 € | |
| Freeze-dried / Lyophilisates | |||
| Saphir Bst2.0 Turbo GreenMaster Lyophilisates | PCR-395S | 192 reactions x 20 µl | 179,20 € |
| PCR-395L | 960 reactions x 20 µl | 716,20 € | |
We also offer bulk amounts of Bst polymerases / mastermixes.
Get in touch for further information at molbio@jenabioscience.com !
