AP₃G (A cap) - Solution

For general laboratory use.

Please centrifuge briefly before opening (volume ≤2 ml).

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.

Shelf Life: 12 months after date of delivery

Molecular Formula: C₂₀H₂₇N₁₀O₁₇P₃ (free acid)

Molecular Weight: 772.41 g/mol (free acid)

Exact Mass: 772.08 g/mol (free acid)

Purity: ≥ 95 % (HPLC)

Form: solution in water

Color: colorless to slightly yellow

Concentration: 100 mM - 110 mM

pH: 7.5 ±0.5

Spectroscopic Properties: λ 259 nm, ε 27.0 L mmol^-1 cm^-1 (Tris-HCl pH 7.5)

Applications:
Synthesis of mRNA with a non-functional cap analog (ApppG) to estimate the level of cap-independent translation.^[1]
Investigation of stress related (Near UV and oxidation) product formation in bacteria^[2-4]

Selected References:
[1] Nowakowska et al. (2014) Cap analogs containing 6-thioguanosine--reagents for the synthesis of mRNAs selectively photo-crosslinkable with cap-binding biomolecules. Org. Biomol. Chem. 12 (27):4841.
[2] Kramer et al. (1988) Near-UV stress in Salmonella typhimurium: 4-thiouridine in tRNA, ppGpp, and ApppGpp as components of an adaptive response. J. Bacteriol. 170 (5):2344.
[3] Bochner et al. (1984) AppppA and related adenylylated nucleotides are synthesized as a consequence of oxidation stress. Cell 37 (1):225.
[4] VanBogelen et al. (1987) Differential induction of heat shock, SOS, and oxidation stress regulons and accumulation of nucleotides in Escherichia coli. J. Bacteriol. 169 (1):26.