Pseudouridine-5'-triphosphate, Sodium Salt
For research use only!
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
Short term exposure (up to 1 week cumulative) to ambient temperature possible.
Shelf Life: 12 months after date of delivery
Molecular Formula: C9H15N2O15P3 (free acid)
Molecular Weight: 484.14 g/mol (free acid)
Exact Mass: 483.97 g/mol (free acid)
Purity: ≥ 95 % (HPLC)
Form: solution in water
Color: colorless to slightly yellow
Concentration: 100 mM - 110 mM
pH: 7.5 ±0.5
Spectroscopic Properties: λmax 265 nm ±2 nm, ε 7.9 L mmol-1 cm-1 (Tris-HCl pH 7.5)
Related products: HighYield T7 RNA Synthesis Kit, #RNT-101
Please click the black arrow on the right to expand the citation list. Click publication title for the full text.
Anderson et al. (2012) Nucleofection induces transient eIF2α phosphorylation by GCN2 and PERK. Gene Ther. doi: 10.1038/gt.2012.5.
Green et al. (2012) Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. J. Biol. Chem. 287 (47):39789.
Karikó et al. (2012) Increased Erythropoiesis in Mice Injected With Submicrogram Quantities of Pseudouridine-containing mRNA Encoding Erythropoietin. Mol Ther. 20 (5):984.
Warren et al. (2012) Feeder-free derivation of human induced pluripotent stem cells with messenger RNA. Sci Rep. 2:657.
Anderson et al. (2011) Nucleoside modifications in RNA limit activation of 2'-5'-oligoadenylate synthetase and increase resistance to cleavage by RNase L. Nucleic Acids Research. 39 (21):9329.
Karikó et al. (2011) Generating the optimal mRNA for therapy: HPLC purification eliminates immune activation and improves translation of nucleoside-modified, protein encoding mRNA. Nucleic Acids Research. 39 (21):e142.
Anderson et al. (2010) Incorporation of pseudouridine into mRNA enhances translation by dimishing PKR activation. Nucleic Acids Research. 38 (17):5884.
Warren et al. (2010) Highly Efficient Reprogramming to Pluripotency and Directed Differentiation of Human Cells with Synthetic Modified mRNA. Cell Stem Cell. doi:10.1016/j.stem.2010.08.012.
Karikó et al. (2008) Incorporation of Pseudouridine into mRNA yields superior nonimmunogenic vector with increased translational capacity and biological stability. Molecular Therapy. 16 (11):1833.
Belliot et al. (2005) Norovirus proteinase-polymerase and polymerase are both active forms of RNA-dependent RNA polymerase. Journal of Virology. 79 (4):2393.
Karikó et al. (2005) Suppression of RNA Recognition by Toll-like Receptors: The Impact of Nucleoside Modifiation and the Evolutionary Origin of RNA. Immunity. 23 (2):165.
Charette et al. (2000) Pseudouridine in RNA: what, where, how, and why. IUBMB Life. 49 (5):341.
Madore et al. (2000) Effect of modified nucleotides on Escherichia coli tRNAGlu structure and on its aminoacylation by glutamyl-tRNA synthetase. Predominant and distinct roles of the mnm5 and s2 modifications of U34. Eur J Biochem. 266 (3):1128.
Goldberg et al. (1963) Comparative utilization of pseudouridine triphosphate and uridine triphosphate by ribonucleic acid polymerase. J. Biol. Chem. 238 (5):1793.