recombinant, E. coli
For in vitro use only!
Unit Definition: One unit is defined as the amount of enzyme required to convert 1 nmol of 5´-[32P]rA16 into a phosphatase-resistant form in 30 minutes at 37°C.
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: ≥ 95 % (SDS-PAGE)
Concentration: 20 units/μl
T4 RNA Ligase 1 catalyzes the ATP-dependent ligation of 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond.
An ideal template is short single-stranded RNA (e.g. miRNA) that can be labeled at the 3'-OH group using labeled pCp as substrate. 3' End Labeling of longer RNA can be achieved with Poly(A) Polymerases.
Recommended final ATP concentration: 1 mM
T4 RNA Ligase 1
#RNT-007-S: 1x 250 μl (20 units/μl)
#RNT-007-L: 4x 250 μl (20 units/μl)
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 50% Glycerol (v/v), pH 7.5
T4 RNA Ligase 1 Reaction Buffer
#RNT-007-S: 1x 1 ml (10x)
#RNT-007-L: 2x 1 ml (10x)
500 mM Tris-HCl (pH 7.5), 100 mM MgCl2, 10 mM DTT
ATP - Solution
1x 100 μl (100 mM)
1x 1.2 ml (50% (w/v))