T4 RNA Ligase 1

For in vitro use only!

Unit Definition: One unit is defined as the amount of enzyme required to convert 1 nmol of 5´-[32P]rA16 into a phosphatase-resistant form in 30 minutes at 37°C.

Shipping: shipped on gel packs

Storage Conditions: store at -20 °C
avoid freeze/thaw cycles

Shelf Life: 12 months

Purity: ≥ 95 % (SDS-PAGE)

Form: liquid

Concentration: 20 units/μl

Description:
T4 RNA Ligase 1 catalyzes the ATP-dependent ligation of 5' phosphoryl-terminated nucleic acid donor to a 3' hydroxyl-terminated nucleic acid acceptor through the formation of a 3'→5' phosphodiester bond.

An ideal template is short single-stranded RNA (e.g. miRNA) that can be labeled at the 3'-OH group using labeled pCp as substrate. 3' End Labeling of longer RNA can be achieved with Poly(A) Polymerases.

Recommended final ATP concentration: 1 mM

Content:
T4 RNA Ligase 1
#RNT-007-S: 1x 250 μl (20 units/μl)
#RNT-007-L: 4x 250 μl (20 units/μl)
10 mM Tris-HCl, 50 mM KCl, 1 mM DTT, 50% Glycerol (v/v), pH 7.5

T4 RNA Ligase 1 Reaction Buffer
#RNT-007-S: 1x 1 ml (10x)
#RNT-007-L: 2x 1 ml (10x)
500 mM Tris-HCl (pH 7.5), 100 mM MgCl₂, 10 mM DTT

ATP - Solution
1x 100 μl (100 mM)

PEG-8000
1x 1.2 ml (50% (w/v))

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• pCp-Cy3, #NU-1706-Cy3
• pCp-Cy5, #NU-1706-Cy5
• pCp-Azide, #NU-1708
• PCR-grade water, #PCR-258

Product Citations:
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