recombinant, E. coli overexpressing E. coli Poly(A) Polymerase
For in vitro use only!
Unit Definition: One unit is defined as the amount of the enzyme required to catalyze the incorporation of 1 nmol of AMP into an acid-insoluble form in 30 minutes at 37 °C.
Shipping: shipped on gel packs
Storage Conditions: store at -20 °C
avoid freeze/thaw cycles
Shelf Life: 12 months
Purity: ≥ 95 % (SDS-PAGE)
Concentration: 5 units/μl
E. coli Poly(A) Polymerase catalyzes the transfer of AMP to 3’-hydroxyl ends of RNA molecules. The reaction is template-independent, requires ATP as substrate and Mg2+as cofactor.
Polyadenylation increases (m)RNA stability and therefore translation efficiency in transfection and microinjection experiments in eukaryotic cells.
For information on (m)RNA polyadenylation using in vitro transcription reaction mixes as template, refer to the Poly(A) Tailing Enzyme Testkit (#RNT-004).
E. coli Poly(A) Polymerase
#RNT-005-S: 1x 100 μl (5 units/μl)
#RNT-005-L: 3x 100 μl (5 units/μl)
25 mM Tris-HCl, 500 mM NaCl, 1 mM MgCl2, 0.1 mM DTT, 0.1 mM EDTA, 50% Glycerol (v/v), pH 8.0
E. coli Poly(A) Polymerase Reaction Buffer
1x 1 ml (10x)
500 mM Tris-HCl (pH 8.0), 2.5 mM NaCl, 100 mM MgCl2
ATP - Solution
1x 100 μl (100 mM)
Related products: PCR-grade water, #PCR-258