Mimic of red fluorescent protein (RFP) fluorophore
For in vitro use only!
Shipping: shipped on blue ice
Storage Conditions: store at -20 °C
Shelf Life: 12 months after date of delivery
Molecular Formula: C12H9F2N3O3
Molecular Weight: 281.22 g/mol
Purity: ≥ 98 % (HPLC)
Form: orange solid
DFHO is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHO-specific RNA sequence (DFHO aptamer) such as Corn.[1-2] DFHO possesses a higher photostability than DFHBI and DFHBI-1T which generally results in a higher signal intensity and lower background in live cell imaging.
Fluorescence is activated upon binding to a matching aptamer sequence:
Exc.max: 505 nm / Em.max: 545 nm / ɛmax: 29000 M-1 cm-1
 Song et al. (2017) Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat. Chem. Biol. 13(11):1187.
 Warner et al. (2017) A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat. Chem. Biol. 13(11):1195.