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DFHO

Mimic of red fluorescent protein (RFP) fluorophore

4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime

Cat. No. Amount Price (EUR) Buy / Note
RNT-003-1 1 mg 130,00 Add to Basket/Quote Add to Notepad
RNT-003-5 5 x 1 mg 390,00 Add to Basket/Quote Add to Notepad
Structural formula of DFHO (Mimic of red fluorescent protein (RFP) fluorophore, 4-(3,5-Difluoro-4-hydroxybenzylidene)-1-methyl-5-oxo-4,5-dihydro-1H-imidazole-2-carbaldehyde oxime)
Structural formula of DFHO

For in vitro use only!

Shipping: shipped on blue ice

Storage Conditions: store at -20 °C
store dark

Shelf Life: 12 months after date of delivery

Molecular Formula: C12H9F2N3O3

Molecular Weight: 281.22 g/mol

CAS#: 1420815-34-4

Purity: ≥ 98 % (HPLC)

Form: orange solid

Solubility: DMSO

Description:
DFHO is cell-permeable and can be used for in vitro and live cell detection of RNA provided with a DFHO-specific RNA sequence (DFHO aptamer) such as Corn.[1-2] DFHO possesses a higher photostability than DFHBI and DFHBI-1T which generally results in a higher signal intensity and lower background in live cell imaging.

Fluorescence is activated upon binding to a matching aptamer sequence:

Corn/DFHO complex[1]:
Exc.max: 505 nm / Em.max: 545 nm / ɛmax: 29000 M-1 cm-1

Selected References:
[1] Song et al. (2017) Imaging RNA polymerase III transcription using a photostable RNA-fluorophore complex. Nat. Chem. Biol. 13(11):1187.
[2] Warner et al. (2017) A homodimer interface without base pairs in an RNA mimic of red fluorescent protein. Nat. Chem. Biol. 13(11):1195.